Fránová-Honetslegrová J, Erbenová M, Martin R R
Department of Plant Virology, Academy of Sciences of the Czech Republic, Ceské Budĕjovice.
Acta Virol. 1998 Nov;42(5):325-31.
Leaves of symptomless Fragaria ananassa Duch cv. Cacanská raná were grafted onto Fragaria vesca indicator clones. Thirty-five of 72 grafted indicator plants developed leaf mottle symptoms. Isometric virus-like particles were observed in purified preparations from symptomatic leaves of F. vesca. The latter were mechanically inoculated to herbaceous host plants. A virus was successfully purified from Nicotiana occidentalis 37 B symptomatic plants by differential and sucrose density gradient centrifugations and a polyclonal antiserum to the virus was prepared. On the basis of serological reactions, symptomatology on herbaceous hosts and electron microscopy studies the virus was identified as tobacco necrosis virus (TNV) D-strain. This is the first isolation of TNV from strawberry leaves and its first finding on strawberry in the Czech Republic. The new experimental hosts N. aucalis, N. bentamiana, N. occidentalis 37 B (systemic hosts), and Ammobium alatum, N. bigelovi, Petunia hybrida (local hosts) for TNV are reported. These results may not exclude the presence of strawberry mottle virus as a causal agent of mottle symptoms in the tested plant samples. Further research is necessary to clarify the aetiology of the strawberry mottle.
将无症状的凤梨草莓品种Cacanská raná的叶片嫁接到野草莓指示克隆植株上。72株嫁接的指示植株中有35株出现了叶片斑驳症状。在从有症状的野草莓叶片中纯化得到的制剂中观察到了等轴状病毒样颗粒。将后者机械接种到草本寄主植物上。通过差速离心和蔗糖密度梯度离心，成功地从感染烟草坏死病毒（TNV）D株系的西方烟草37B有症状植株中纯化出一种病毒，并制备了针对该病毒的多克隆抗血清。基于血清学反应、草本寄主上的症状学以及电子显微镜研究，该病毒被鉴定为烟草坏死病毒（TNV）D株系。这是首次从草莓叶片中分离出TNV，也是在捷克共和国首次在草莓上发现该病毒。报道了TNV的新实验寄主，即澳洲烟草、本氏烟草、西方烟草37B（系统寄主），以及翼柄银苞菊、比格洛烟草、矮牵牛（局部寄主）。这些结果并不排除草莓斑驳病毒作为测试植物样本中斑驳症状致病因子的存在。有必要进一步研究以阐明草莓斑驳病的病因。
