人乳头瘤病毒16 E7在转化过程中的核内定位以及E7与Rb家族成员p130的优先结合
Intranuclear localization of human papillomavirus 16 E7 during transformation and preferential binding of E7 to the Rb family member p130.
作者信息
Smith-McCune K, Kalman D, Robbins C, Shivakumar S, Yuschenkoff L, Bishop J M
机构信息
Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco CA 94115, USA.
出版信息
Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6999-7004. doi: 10.1073/pnas.96.12.6999.
Abstract
To study intracellular pathways by which the human papillomavirus 16 oncogene E7 participates in carcinogenesis, we expressed an inducible chimera of E7 by fusion to the hormone-binding domain of the estrogen receptor. The chimeric protein (E7ER) transformed rodent fibroblast cell lines and induced DNA synthesis on addition of estradiol. In coimmunoprecipitation experiments, E7ER preferentially bound p130 when compared to p107 and pRb. After estradiol addition, E7ER localization changed to a more intense intranuclear staining. Induction of E7 function was not correlated with binding to p130 or pRb but rather with intranuclear localization and modest induction of binding to p107.
摘要
为了研究人乳头瘤病毒16型癌基因E7参与致癌作用的细胞内途径，我们通过与雌激素受体的激素结合域融合表达了一种可诱导的E7嵌合体。这种嵌合蛋白（E7ER）转化了啮齿动物成纤维细胞系，并在添加雌二醇后诱导DNA合成。在免疫共沉淀实验中，与p107和pRb相比，E7ER优先结合p130。添加雌二醇后，E7ER的定位改变为更强的核内染色。E7功能的诱导与与p130或pRb的结合无关，而是与核内定位以及与p107结合的适度诱导有关。
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Differential expression and functionally co-operative roles for the retinoblastoma family of proteins in epidermal differentiation.视网膜母细胞瘤蛋白家族在表皮分化中的差异表达及功能协同作用。
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