在功能完整的视网膜中对光诱发钙信号进行光学记录。
Optical recording of light-evoked calcium signals in the functionally intact retina.
作者信息
Denk W, Detwiler P B
机构信息
Bell Laboratories, Lucent Technologies, Murray Hill, NJ 07974, USA.
出版信息
Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):7035-40. doi: 10.1073/pnas.96.12.7035.
Abstract
Using two-photon excitation of fluorescent indicator dyes, we measured calcium concentration transients in retinal ganglion and amacrine cells without destroying the light sensitivity of the retina by maximally activating or bleaching the photoreceptors. This allowed an immediate assessment of the cellular morphology and study of the calcium signals evoked by visual stimuli. Calcium dynamics in individual dendritic processes could be examined for extensive periods without deterioration and with little apparent phototoxicity at excitation wavelengths of from 930 to 990 nm. Light-evoked increases in calcium were resolved in ganglion- and amacrine-cell neurites, making it possible to use optical recording to study the relationship between calcium signaling and retinal function.
摘要
我们使用荧光指示剂染料的双光子激发技术，测量了视网膜神经节细胞和无长突细胞中的钙浓度瞬变，在不通过最大程度激活或漂白光感受器而破坏视网膜光敏感性的情况下进行测量。这使得能够立即评估细胞形态，并研究视觉刺激诱发的钙信号。在930至990nm的激发波长下，可以长时间检查单个树突状突起中的钙动力学，而不会恶化，且几乎没有明显的光毒性。在神经节细胞和无长突细胞的神经突中分辨出光诱发的钙增加，这使得利用光学记录来研究钙信号传导与视网膜功能之间的关系成为可能。
相似文献
1
Optical recording of light-evoked calcium signals in the functionally intact retina.
Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):7035-40. doi: 10.1073/pnas.96.12.7035.
2
Calcium increases in retinal glial cells evoked by light-induced neuronal activity.
J Neurosci. 2005 Jun 8;25(23):5502-10. doi: 10.1523/JNEUROSCI.1354-05.2005.
3
Eyecup scope--optical recordings of light stimulus-evoked fluorescence signals in the retina.
Pflugers Arch. 2009 Apr;457(6):1393-414. doi: 10.1007/s00424-008-0603-5. Epub 2008 Nov 21.
4
Inhibitory Interneurons in the Retina: Types, Circuitry, and Function.
Annu Rev Vis Sci. 2017 Sep 15;3:1-24. doi: 10.1146/annurev-vision-102016-061345. Epub 2017 Jun 15.
5
Effect of spike blockade on the receptive-field size of amacrine and ganglion cells in the rabbit retina.
J Neurophysiol. 1996 May;75(5):1878-93. doi: 10.1152/jn.1996.75.5.1878.
6
Intrinsically photosensitive retinal ganglion cells.
Sci China Life Sci. 2010 Jan;53(1):58-67. doi: 10.1007/s11427-010-0024-5. Epub 2010 Feb 12.
7
The organization of the turtle inner retina. II. Analysis of color-coded and directionally selective cells.
J Comp Neurol. 1995 Jul 17;358(1):35-62. doi: 10.1002/cne.903580103.
8
Electrophysiological characterization of GFP-expressing cell populations in the intact retina.
J Vis Exp. 2011 Nov 14(57):3457. doi: 10.3791/3457.
9
[Viral and Electrophysiological Approaches for Elucidating the Structure and Function of Retinal Circuits].
Yakugaku Zasshi. 2018;138(5):669-678. doi: 10.1248/yakushi.17-00200-1.
10
The organization of the turtle inner retina. I. ON- and OFF-center pathways.
J Comp Neurol. 1995 Jul 17;358(1):1-34. doi: 10.1002/cne.903580102.
引用本文的文献
1
Subcellular pathways through VGluT3-expressing mouse amacrine cells provide locally tuned object-motion-selective signals in the retina.
Nat Commun. 2024 Apr 5;15(1):2965. doi: 10.1038/s41467-024-46996-0.
2
More than double the fun with two-photon excitation microscopy.
Commun Biol. 2024 Mar 26;7(1):364. doi: 10.1038/s42003-024-06057-0.
3
Cortical Representation of Touch in Silico.
Neuroinformatics. 2022 Oct;20(4):1013-1039. doi: 10.1007/s12021-022-09576-5. Epub 2022 Apr 29.
4
Probing Light-Stimulated Activities in the Retina via Transparent Graphene Electrodes.
ACS Appl Bio Mater. 2022 Jan 17;5(1):305-312. doi: 10.1021/acsabm.1c01091. Epub 2022 Jan 6.
5
Micelle-enabled self-assembly of porous and monolithic carbon membranes for bioelectronic interfaces.
Nat Nanotechnol. 2021 Feb;16(2):206-213. doi: 10.1038/s41565-020-00805-z. Epub 2020 Dec 7.
6
Seeing Natural Images through the Eye of a Fly with Remote Focusing Two-Photon Microscopy.
iScience. 2020 Jun 26;23(6):101170. doi: 10.1016/j.isci.2020.101170. Epub 2020 May 17.
7
Flexible Nanopipettes for Minimally Invasive Intracellular Electrophysiology In Vivo.
Cell Rep. 2019 Jan 2;26(1):266-278.e5. doi: 10.1016/j.celrep.2018.12.019.
8
Localizing Proton-Mediated Inhibitory Feedback at the Retinal Horizontal Cell-Cone Synapse with Genetically-Encoded pH Probes.
J Neurosci. 2019 Jan 23;39(4):651-662. doi: 10.1523/JNEUROSCI.1541-18.2018. Epub 2018 Nov 30.
9
Correction-free remotely scanned two-photon mouse retinal imaging.
Light Sci Appl. 2016 Jan 1;5(1):e16007. doi: 10.1038/lsa.2016.7. eCollection 2016 Jan.
10
A Dense Starburst Plexus Is Critical for Generating Direction Selectivity.
Curr Biol. 2018 Apr 23;28(8):1204-1212.e5. doi: 10.1016/j.cub.2018.03.001. Epub 2018 Mar 29.
本文引用的文献
1
Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins.
Nature. 1998 Jul 9;394(6689):192-5. doi: 10.1038/28190.
2
Translocation of calmodulin to the nucleus supports CREB phosphorylation in hippocampal neurons.
Nature. 1998 Mar 12;392(6672):198-202. doi: 10.1038/32448.
3
Bleached pigment produces a maintained decrease in outer segment Ca2+ in salamander rods.
J Gen Physiol. 1998 Jan;111(1):53-64. doi: 10.1085/jgp.111.1.53.
4
A genetically encoded optical probe of membrane voltage.
Neuron. 1997 Oct;19(4):735-41. doi: 10.1016/s0896-6273(00)80955-1.
5
Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin.
Nature. 1997 Aug 28;388(6645):882-7. doi: 10.1038/42264.
6
Detection in living cells of Ca2+-dependent changes in the fluorescence emission of an indicator composed of two green fluorescent protein variants linked by a calmodulin-binding sequence. A new class of fluorescent indicators.
J Biol Chem. 1997 May 16;272(20):13270-4. doi: 10.1074/jbc.272.20.13270.
7
The light response of retinal ganglion cells is truncated by a displaced amacrine circuit.
Neuron. 1997 Apr;18(4):637-50. doi: 10.1016/s0896-6273(00)80304-9.
8
Photon upmanship: why multiphoton imaging is more than a gimmick.
Neuron. 1997 Mar;18(3):351-7. doi: 10.1016/s0896-6273(00)81237-4.
9
In vivo dendritic calcium dynamics in neocortical pyramidal neurons.
Nature. 1997 Jan 9;385(6612):161-5. doi: 10.1038/385161a0.
10
Calcium imaging of single stereocilia in hair cells: localization of transduction channels at both ends of tip links.
Neuron. 1995 Dec;15(6):1311-21. doi: 10.1016/0896-6273(95)90010-1.
Zotero 插件