在功能完整的视网膜中对光诱发钙信号进行光学记录。
Optical recording of light-evoked calcium signals in the functionally intact retina.
作者信息
Denk W, Detwiler P B
机构信息
Bell Laboratories, Lucent Technologies, Murray Hill, NJ 07974, USA.
出版信息
Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):7035-40. doi: 10.1073/pnas.96.12.7035.
Abstract
Using two-photon excitation of fluorescent indicator dyes, we measured calcium concentration transients in retinal ganglion and amacrine cells without destroying the light sensitivity of the retina by maximally activating or bleaching the photoreceptors. This allowed an immediate assessment of the cellular morphology and study of the calcium signals evoked by visual stimuli. Calcium dynamics in individual dendritic processes could be examined for extensive periods without deterioration and with little apparent phototoxicity at excitation wavelengths of from 930 to 990 nm. Light-evoked increases in calcium were resolved in ganglion- and amacrine-cell neurites, making it possible to use optical recording to study the relationship between calcium signaling and retinal function.
摘要
我们使用荧光指示剂染料的双光子激发技术，测量了视网膜神经节细胞和无长突细胞中的钙浓度瞬变，在不通过最大程度激活或漂白光感受器而破坏视网膜光敏感性的情况下进行测量。这使得能够立即评估细胞形态，并研究视觉刺激诱发的钙信号。在930至990nm的激发波长下，可以长时间检查单个树突状突起中的钙动力学，而不会恶化，且几乎没有明显的光毒性。在神经节细胞和无长突细胞的神经突中分辨出光诱发的钙增加，这使得利用光学记录来研究钙信号传导与视网膜功能之间的关系成为可能。
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