Marangoni A, Sambri V, Olmo A, D'Antuono A, Negosanti M, Cevenini R
Sezione di Microbiologia, Policlinico S. Orsola, University of Bologna, Italy.
Zentralbl Bakteriol. 1999 Apr;289(2):125-33. doi: 10.1016/s0934-8840(99)80095-4.
Sensitivity and specificity of IgG detection by Western blotting performed with a lysate of Treponema pallidum whole cells were compared with those of the most common assays used in the laboratory diagnosis of syphilis, i.e. fluorescent treponemal antibody absorption test (FTA-ABS) and treponemal haemagglutination assay (TPHA). Thirty-five serum samples obtained from twenty-one patients with a clinical diagnosis of early syphilis, based on the presence of typical chancre or skin or mucous membrane lesions, were studied. In addition, thirty blood samples from donors, ten sera positive for Borrelia burgdorferi and five positive for Leptospira interrogans were tested as controls. The clinical diagnosis was the reference method used to compare the performance of the serological tests. Western blotting performed with a sensitivity and specificity of 100%, whereas the corresponding sensitivity and specificity for FTA-ABS were 88.5% and 98%, respectively. The performance of TPHA showed a sensitivity of 86% and a specificity of 100%.
将用梅毒螺旋体全细胞裂解物进行蛋白质印迹法检测IgG的灵敏度和特异性，与梅毒实验室诊断中最常用的检测方法，即荧光密螺旋体抗体吸收试验（FTA-ABS）和密螺旋体血凝试验（TPHA）的灵敏度和特异性进行比较。研究了从21例临床诊断为早期梅毒的患者中获得的35份血清样本，这些患者基于典型溃疡或皮肤或粘膜病变的存在进行诊断。此外，还检测了来自献血者的30份血液样本、10份伯氏疏螺旋体血清阳性样本和5份问号钩端螺旋体血清阳性样本作为对照。临床诊断是用于比较血清学检测性能的参考方法。蛋白质印迹法的灵敏度和特异性均为100%，而FTA-ABS的相应灵敏度和特异性分别为88.5%和98%。TPHA的性能显示灵敏度为86%，特异性为100%。
