Marangoni Antonella, Moroni Alessandra, Accardo Silvia, Cevenini Roberto
Dipartimento di Ematologia e Sc. Oncologiche, Section of Microbiology, St. Orsola Hospital, University of Bologna, Bologna, Italy.
J Clin Lab Anal. 2009;23(1):1-6. doi: 10.1002/jcla.20268.
The serological detection of specific antibodies to Treponema pallidum is of particular importance in the diagnosis of syphilis. The purpose of this study was to evaluate diagnostic performances of automated immunoassays in comparison with T. pallidum hemagglutination test (TPHA) and Western Blot (WB). The retrospective study was performed with different panels of sera: 244 clinical and serological characterized syphilitic sera and 203 potentially interfering samples. All the sera were tested by Enzygnost Syphilis, ARCHITECT Syphilis TP, TPHA, and homemade WB. The diagnostic performances of the two assays were very similar: both Enzygnost Syphilis and ARCHITECT Syphilis TP performed with a sensitivity of 99.2%, whereas the specificity was 98.5 and 98.4%, respectively. Considering the suitability for automation, both immunoassays may represent a good choice as a screening test. However, the use of a confirmatory test, such as TPHA or WB, remains a must in order to avoid false-positive results.
梅毒螺旋体特异性抗体的血清学检测在梅毒诊断中尤为重要。本研究的目的是评估自动化免疫分析与梅毒螺旋体血凝试验（TPHA）和免疫印迹法（WB）相比的诊断性能。本回顾性研究使用了不同的血清样本组：244份具有临床和血清学特征的梅毒血清以及203份可能产生干扰的样本。所有血清均采用酶联免疫吸附试验梅毒检测试剂（Enzygnost Syphilis）、化学发光微粒子免疫检测梅毒螺旋体抗体试剂（ARCHITECT Syphilis TP）、TPHA和自制的WB进行检测。这两种检测方法的诊断性能非常相似：酶联免疫吸附试验梅毒检测试剂和化学发光微粒子免疫检测梅毒螺旋体抗体试剂的敏感性均为99.2%，而特异性分别为98.5%和98.4%。考虑到自动化的适用性，这两种免疫分析方法作为筛查试验可能都是不错的选择。然而，为了避免假阳性结果，使用确证试验，如TPHA或WB，仍然是必要的。
