高效液相色谱法测定小牛血清中（±）-乳酸及其对映体
High-performance liquid chromatographic assay of (+/-)-lactic acid and its enantiomers in calf serum.
作者信息
Omole O O, Brocks D R, Nappert G, Naylor J M, Zello G A
机构信息
College of Pharmacy and Nutrition and The Department of Internal Medicine, University of Saskatchewan, Saskatoon, Canada.
出版信息
J Chromatogr B Biomed Sci Appl. 1999 Apr 30;727(1-2):23-9. doi: 10.1016/s0378-4347(99)00072-9.
Two high-performance liquid chromatographic methods are described for the determination of lactic acid and its enantiomers in calf serum. A 300x8.0 mm I.D. column packed with sulfonated styrene-divinylbenzene copolymer and a 50x4.6 mm ODS column with N,N-dioctyl-L-alanine were used. UV detection was at 205 and 236 nm for the non-chiral and chiral assays, respectively. Both assays demonstrated excellent linear relationships between peak area ratios and serum concentrations over a range of 0.5 to 20 mM, based on 100 microl bovine serum. Recovery was complete. Inter- and within-batch bias and relative standard deviation were <15%.
描述了两种用于测定小牛血清中乳酸及其对映体的高效液相色谱方法。使用了内径为8.0 mm、长300 mm填充有磺化苯乙烯 - 二乙烯基苯共聚物的色谱柱以及内径为4.6 mm、长50 mm填充有N,N - 二辛基 - L - 丙氨酸的ODS柱。非手性和手性测定的紫外检测波长分别为205 nm和236 nm。基于100微升牛血清，两种测定方法在0.5至20 mM范围内峰面积比与血清浓度之间均显示出良好的线性关系。回收率完全。批间和批内偏差以及相对标准偏差均<15%。
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