Lúdvíksdóttir D, Janson C, Högman M, Gudbjörnsson B, Björnsson E, Valtýsdóttir S, Hedenström H, Venge P, Boman G
Dept of Lung Medicine, Akademiska sjukhuset, Uppsala University, Sweden.
Eur Respir J. 1999 Apr;13(4):739-43. doi: 10.1034/j.1399-3003.1999.13d07.x.
Nitric oxide has an important role in the regulation of airway function and can have pro-inflammatory effects. Bronchial hyperresponsiveness (BHR) and respiratory symptoms are common in patients with Sjögren's syndrome (SS). The aim of this study was to determine whether patients with SS have an increased amount of exhaled NO and whether this NO correlates with respiratory symptoms and BHR. Exhaled NO was measured in 18 patients with SS and 13 normal subjects on three different occasions with intervals of at least 3 days using a chemiluminescence method. Airway responsiveness was assessed with methacholine provocation. Serum levels of myeloperoxidase (MPO), human neutrophil lipocalin (HNL), eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO) were measured. Exhaled NO was significantly higher in patients with SS than in controls (147+/-82 versus 88+/-52 nL x min(-1); mean+/-SD; p=0.041). Exhaled NO was correlated with age (partial r=0.52, p=0.006) and serum HNL (partial r=0.46, p=0.014). There were no significant correlations between exhaled NO and respiratory symptoms, BHR or serum MPO, ECP or EPO. Disease duration was negatively associated with serum MPO (r=-0.47, p=0.043). In patients with SS, a positive correlation was found between symptom score and serum ECP (partial r=0.65, p=0.003) and EPO (partial r=0.62, p=0.004) and a negative correlation with age (partial r=-0.60, p=0.005). In conclusion, elevated levels of exhaled nitric oxide in patients with Sjögren's syndrome were demonstrated. The mechanism underlying this increase in exhaled nitric oxide in Sjögren's syndrome is not known.
一氧化氮在气道功能调节中发挥着重要作用，并且可能具有促炎作用。支气管高反应性（BHR）和呼吸道症状在干燥综合征（SS）患者中很常见。本研究的目的是确定SS患者呼出的一氧化氮量是否增加，以及这种一氧化氮是否与呼吸道症状和BHR相关。使用化学发光法，在18例SS患者和13名正常受试者身上，于三个不同时间点（间隔至少3天）测量呼出的一氧化氮。通过乙酰甲胆碱激发试验评估气道反应性。测量血清髓过氧化物酶（MPO）、人中性粒细胞脂质运载蛋白（HNL）、嗜酸性粒细胞阳离子蛋白（ECP）和嗜酸性粒细胞过氧化物酶（EPO）的水平。SS患者呼出的一氧化氮显著高于对照组（147±82 vs 88±52 nL·min⁻¹；均值±标准差；p = 0.041）。呼出的一氧化氮与年龄相关（偏相关系数r = 0.52，p = 0.006）和血清HNL相关（偏相关系数r = 0.46，p = 0.014）。呼出的一氧化氮与呼吸道症状、BHR或血清MPO、ECP或EPO之间无显著相关性。疾病持续时间与血清MPO呈负相关（r = -0.47，p = 0.043）。在SS患者中，症状评分与血清ECP（偏相关系数r = 0.65，p = 0.003）和EPO（偏相关系数r = 0.62，p = 0.004）呈正相关，与年龄呈负相关（偏相关系数r = -0.60，p = 0.005）。总之，已证实干燥综合征患者呼出的一氧化氮水平升高。干燥综合征患者呼出一氧化氮增加的潜在机制尚不清楚。
