Wu H L, Hsu C Y, Liu W H, Yung B Y
Department of Pharmacology, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan, Republic of China.
Int J Cancer. 1999 Jun 11;81(6):923-9. doi: 10.1002/(sici)1097-0215(19990611)81:6<923::aid-ijc14>3.0.co;2-d.
The steady-state level of nucleophosmin/B23 mRNA decreased during berberine-induced (25 microg/ml, 24 to 96 hr) apoptosis of human leukemia HL-60 cells. A decline in telomerase activity was also observed in HL-60 cells treated with berberine. A stable clone of nucleophosmin/B23 overexpressed in HL-60 cells was selected and found to be less responsive to berberine-induced apoptosis. About 35% to 63% of control vector-transfected cells (pCR3) exhibited morphological characteristics of apoptosis, while about 8% to 45% of nucleophosmin/B23-over-expressed cells (pCR3-B23) became apoptotic after incubation with 15 microg/ml berberine for 48 to 96 hr. DNA extracted from pCR3 cells contained more fragmented DNA than pCR3-B23 cells during treatment with 15 microg/ml berberine for 24 to 48 hr. Our results indicate that berberine-induced apoptosis is associated with down-regulation of nucleophosmin/B23 and telomerase activity. We also suggest that nucleophosmin/B23 may play an important role in the control of the cellular response to apoptosis induction.
在小檗碱诱导（25微克/毫升，24至96小时）人白血病HL-60细胞凋亡的过程中，核磷蛋白/B23信使核糖核酸的稳态水平下降。在用小檗碱处理的HL-60细胞中也观察到端粒酶活性降低。筛选出一个在HL-60细胞中过表达核磷蛋白/B23的稳定克隆，发现其对小檗碱诱导的凋亡反应较弱。约35%至63%的对照载体转染细胞（pCR3）呈现出凋亡的形态特征，而在用15微克/毫升小檗碱孵育48至96小时后，约8%至45%的核磷蛋白/B23过表达细胞（pCR3-B23）发生凋亡。在用15微克/毫升小檗碱处理24至48小时期间，从pCR3细胞中提取的DNA比pCR3-B23细胞含有更多的片段化DNA。我们的结果表明，小檗碱诱导的凋亡与核磷蛋白/B23和端粒酶活性的下调有关。我们还认为，核磷蛋白/B23可能在控制细胞对凋亡诱导的反应中起重要作用。
