Nobin A, Baumgarten H G, Falck B, Ingemansson S, Moghimzadeh E, Rosengren E
Cell Tissue Res. 1978 Dec 29;195(3):371-80. doi: 10.1007/BF00233883.
The sympathetic innervation of the liver of monkey and man has been investigated in a combined fluorescence histochemical, chemical and electron microscopical study. By means of the Falck-Hillarp fluorescence method a dense network of monoamine-containing nerve fibers was visualized in liver tissue of monkey and man. The nerve fibers ran in close contact to both hepatocytes and blood vessels. Chemical quantitations showed high concentrations of noradrenaline in both human and monkey liver. Microspectrofluorometry of the intraneuronal monoamine resulted in spectra characteristic of a catecholamine. For the electron microscopical study the dopamine analogue, 5-hydroxydopamine, was used to "label" the catecholamine terminals in both human and monkey liver. The nerve profiles, identified as catecholamine-containing, were demonstrated in a perivascular location and in close contact to hepatocytes. No synaptic membrane specializations were present between nerve fibers and hepatocytes. The general ultramorphology and intralobular distribution pattern of nerves in the liver of monkey and man were similar. The present results prove the existence of a sympathetic innervation of hepatocytes and blood vessels in the liver of man and monkey.
通过荧光组织化学、化学和电子显微镜联合研究，对猴和人的肝脏交感神经支配进行了调查。借助福尔克-希拉尔普荧光法，在猴和人的肝脏组织中可见到密集的含单胺神经纤维网络。神经纤维与肝细胞和血管紧密接触。化学定量分析表明，人和猴的肝脏中去甲肾上腺素浓度都很高。对神经元内单胺进行显微分光荧光测定得到了儿茶酚胺特有的光谱。在电子显微镜研究中，多巴胺类似物5-羟多巴胺被用于“标记”人和猴肝脏中的儿茶酚胺终末。被鉴定为含儿茶酚胺的神经轮廓见于血管周围位置，并与肝细胞紧密接触。神经纤维与肝细胞之间不存在突触膜特化结构。猴和人肝脏中神经的一般超微形态和小叶内分布模式相似。目前的结果证明，人和猴的肝脏中存在对肝细胞和血管的交感神经支配。
