Tsantarliotou M, Taitzoglou I, Kokolis N
Department of Physiology, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Greece.
In Vivo. 1999 Mar-Apr;13(2):119-24.
Dexamethasone in low and high doses affects blood fibrinolytic activity both in animals and humans. In this study the effect of a high dose of dexamethasone on plasminogen activator activity (PAA), t-PA antigen level, plasminogen activator inhibition (PAI) and plasmin inhibition (Pl) in rat heart, brain, liver, lungs and kidneys was investigated in both sexes.
Twenty male and twenty female adult Wistar rats were used. Dexamethasone was administered as a single intraperitoneal injection (3mg/kg/day) in rats, once daily, for a period of 5 consecutive days. t-PA antigen level was assayed by an enzyme-linked immunoabsorbant assay method. PAA, PAI and Pl were determined by spectrophotometric methods. The plasminogen used was isolated from rat plasma.
Dexamethasone induced variable changes in the fibrinolytic parameters in rat heart, brain and liver of both sexes; in lungs and kidneys dexamethasone had no effect.
These changes of PAA, PAI and t-PA antigen level in heart, brain and liver induced by dexamethasone might be of importance regarding the involvement of glucocorticoids and plasminogen activators/plasmin in many pathophysiological conditions.
低剂量和高剂量地塞米松对动物和人类的血液纤维蛋白溶解活性均有影响。在本研究中，对雄性和雌性大鼠的心脏、大脑、肝脏、肺和肾脏中高剂量地塞米松对纤溶酶原激活物活性（PAA）、组织型纤溶酶原激活物抗原水平（t-PA）、纤溶酶原激活物抑制物（PAI）和纤溶酶抑制物（Pl）的影响进行了研究。
使用20只雄性和20只雌性成年Wistar大鼠。大鼠腹腔注射地塞米松（3mg/kg/天），每日1次，连续5天。采用酶联免疫吸附测定法检测t-PA抗原水平。通过分光光度法测定PAA、PAI和Pl。所用纤溶酶原从大鼠血浆中分离。
地塞米松在雄性和雌性大鼠的心脏、大脑和肝脏中诱导了纤维蛋白溶解参数的不同变化；在肺和肾脏中，地塞米松没有影响。
地塞米松诱导的心脏、大脑和肝脏中PAA、PAI和t-PA抗原水平的这些变化，可能在糖皮质激素和纤溶酶原激活物/纤溶酶参与多种病理生理状况方面具有重要意义。
