Ikić D, Beck M, Turner V
Dev Biol Stand. 1976;37:235-40.
The subcutis of 11-day-old chick embryos, 15- to 18-day-old mouse embryos and the skin muscle tissue of human fetus were stretched on lens paper in a Petri dish and seeded with a piece of LED-Widr or HDC cell sheet. Mem + 10% FCS was added. After three days incubation the material was fixed, sectioned and H-E stained. In the second part of our investigation several cell lines were prepared on cover slips. When confluent, the cultures were inoculated with 1-2 drops of cell suspension of another cell line. Three days after the seeding of cell suspension on the confluent cell sheet the cultures were fixed and stained. It can be seen in the organ cultures that LED-Widr cells closely adhere to the embryonic tissue which in some places they are invading. In tissue culture examination we found that heteroploid cells seeded on a confluent sheet of diploid cells adhered but that a diploid cell seeded on epithelial-like heteroploid cells would not adhere. The testing of invasiveness in embryonic tissue in organ culture showed that it can be a useful model. By seeding suspended cells of fibroblast lines on a confluent sheet of heteroploid cell lines their contact inhibition or their invasive ability can be determined. The seeding of heteroploid cell lines on diploid cell lines can be used as a model for the study of the mechanism of invasion into normal tissue and for the in vitro study of invasion inhibiting properties of certain substances.
将11日龄鸡胚的皮下组织、15至18日龄小鼠胚胎的皮下组织以及人胎儿的皮肤肌肉组织铺展在培养皿中的镜头纸上，接种一片LED-Widr或HDC细胞片。加入含10%胎牛血清的培养基。孵育三天后，将材料固定、切片并进行苏木精-伊红染色。在我们研究的第二部分，在盖玻片上制备了几种细胞系。当细胞汇合时，用另一种细胞系的1-2滴细胞悬液接种培养物。在汇合细胞片上接种细胞悬液三天后，将培养物固定并染色。在器官培养中可以看到，LED-Widr细胞紧密附着于胚胎组织，在某些地方它们还侵入了胚胎组织。在组织培养检查中，我们发现接种在汇合二倍体细胞片上的异倍体细胞会附着，但接种在上皮样异倍体细胞上的二倍体细胞不会附着。在器官培养中对胚胎组织侵袭性的测试表明，它可以是一个有用的模型。通过将成纤维细胞系的悬浮细胞接种在汇合的异倍体细胞系片上，可以确定它们的接触抑制或侵袭能力。将异倍体细胞系接种在二倍体细胞系上可作为研究侵入正常组织机制以及某些物质体外侵袭抑制特性的模型。
