突触NMDA受体激活后AMPA受体的快速脊柱传递与重新分布。

Rapid spine delivery and redistribution of AMPA receptors after synaptic NMDA receptor activation.

作者信息

Shi S H, Hayashi Y, Petralia R S, Zaman S H, Wenthold R J, Svoboda K, Malinow R

机构信息

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

出版信息

Science. 1999 Jun 11;284(5421):1811-6. doi: 10.1126/science.284.5421.1811.

DOI:10.1126/science.284.5421.1811

PMID:10364548

Abstract

To monitor changes in alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor distribution in living neurons, the AMPA receptor subunit GluR1 was tagged with green fluorescent protein (GFP). This protein (GluR1-GFP) was functional and was transiently expressed in hippocampal CA1 neurons. In dendrites visualized with two-photon laser scanning microscopy or electron microscopy, most of the GluR1-GFP was intracellular, mimicking endogenous GluR1 distribution. Tetanic synaptic stimulation induced a rapid delivery of tagged receptors into dendritic spines as well as clusters in dendrites. These postsynaptic trafficking events required synaptic N-methyl-D-aspartate (NMDA) receptor activation and may contribute to the enhanced AMPA receptor-mediatedtransmission observed during long-term potentiation and activity-dependent synaptic maturation.

摘要

为监测活神经元中α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体分布的变化，AMPA受体亚基GluR1用绿色荧光蛋白（GFP）进行标记。该蛋白（GluR1-GFP）具有功能，并在海马CA1神经元中瞬时表达。在用双光子激光扫描显微镜或电子显微镜观察的树突中，大多数GluR1-GFP位于细胞内，这与内源性GluR1的分布相似。强直突触刺激诱导标记的受体迅速转运到树突棘以及树突中的簇中。这些突触后转运事件需要突触N-甲基-D-天冬氨酸（NMDA）受体激活，并且可能有助于在长时程增强和活动依赖性突触成熟过程中观察到的AMPA受体介导的传递增强。