一株来自英国的具有缺失ORF 3a的新型强毒传染性胃肠炎病毒的特性分析
Characterisation of a recent virulent transmissible gastroenteritis virus from Britain with a deleted ORF 3a.
作者信息
McGoldrick A, Lowings J P, Paton D J
机构信息
Virology Department, Veterinary Laboratories Agency, Addlestone, Surrey, U.K.
出版信息
Arch Virol. 1999;144(4):763-70. doi: 10.1007/s007050050541.
Abstract
Analyses of transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) isolates have suggested that tropism and pathogenicity are influenced by the spike protein and ORF 3. In general, enteric viruses (TGEV) have been shown to contain intact spike and ORF 3 genes, whilst respiratory isolates (PRCV) have major deletions within both regions. Virulence has been correlated to a functional ORF 3. Here, sequence analysis of a recent isolate of virulent TGEV, revealed a variant with an intact spike gene, but a large deletion in ORF 3a. This suggests that ORF 3a is not essential for enteric virulence.
摘要
对传染性胃肠炎病毒（TGEV）和猪呼吸道冠状病毒（PRCV）分离株的分析表明，嗜性和致病性受刺突蛋白和开放阅读框3（ORF 3）影响。一般来说，肠道病毒（TGEV）已被证明含有完整的刺突和ORF 3基因，而呼吸道分离株（PRCV）在这两个区域都有主要缺失。毒力与功能性ORF 3相关。在此，对一株近期强毒株TGEV的序列分析显示，有一个变体具有完整的刺突基因，但ORF 3a有一个大的缺失。这表明ORF 3a对于肠道毒力并非必不可少。
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