Veau B, Guillot J, Damez M, Dusser M, Konska G, Botton B
Department of Botany, Cryptogamy and Microbiology, Faculty of Pharmaceutical Sciences, University of Clermont I, 28 place Henri Dunant, 63100, Clermont-Ferrand, France.
Biochim Biophys Acta. 1999 Jun 28;1428(1):39-44. doi: 10.1016/s0304-4165(99)00055-0.
A lectin (HHL) was isolated from the fruiting body of the mushroom Hygrophorus hypothejus by a combination of affinity chromatography on stromas of group B erythrocytes embedded in polyacrylamide gel, and DEAE-trisacryl and gel filtration chromatography. Its molecular mass, as determined by gel filtration, is estimated to be 68000 kDa and its structure is tetrameric with four identical subunits assembled with non-covalent bonds. HHL agglutinates specifically A and B blood group erythrocytes and in hemagglutination inhibition assays, exhibits sugar-binding specificity toward lactose, the anomeric alpha form being more effective than the beta form.
