美味乳菇中一种凝集素的分离与鉴定

Isolation and characterization of a lectin from the mushroom, Lactarius deliciosus.

作者信息

Guillot J, Giollant M, Damez M, Dusser M

机构信息

Department of Botany and Microbiology, Faculty of Pharmaceutical Sciences, University of Clermont I, Clermont-Ferrand, France.

出版信息

J Biochem. 1991 Jun;109(6):840-5. doi: 10.1093/oxfordjournals.jbchem.a123468.

DOI:10.1093/oxfordjournals.jbchem.a123468

PMID:1939003

Abstract

A lectin (LDL) has been isolated from carpophores of the edible mushroom, Lactarius deliciosus, using a combination of affinity chromatography on stromas of group O erythrocytes embedded in polyacrylamide gel and hydroxylapatite, and gel filtration chromatography. Its molecular weight, as determined by gel filtration, is about 37,000 and its structure is dimeric, with two distinct types of subunits (about 19,000 and 18,000). It appeared homogeneous on HPLC gel filtration, but exhibited microheterogeneity on isoelectric focusing. Amino acid analysis revealed that it contains a large amount of glycine. Hapten inhibition assaying indicated that the Lactarius lectin is most specific for D-Gal beta 1----3D-GalNAc. The lectin was found in the mycelium and its possible role in the fungus is discussed.

摘要

通过将包埋于聚丙烯酰胺凝胶中的O型红细胞基质与羟基磷灰石进行亲和层析，再结合凝胶过滤层析，从美味乳菇的子实体中分离出一种凝集素（LDL）。经凝胶过滤测定，其分子量约为37,000，结构为二聚体，有两种不同类型的亚基（约19,000和18,000）。在高效液相色谱凝胶过滤中它显得均一，但在等电聚焦时表现出微不均一性。氨基酸分析表明它含有大量甘氨酸。半抗原抑制测定表明，乳菇凝集素对D-Galβ1----3D-GalNAc最为特异。在菌丝体中发现了这种凝集素，并讨论了其在真菌中的可能作用。