Zhou M, Chen Y, Liu S, Ding Z, Pang Z, Wan J
Research Laboratory of Free Radical Medicine, First Military Medical University, Guangzhou, People's Republic of China.
Br J Biomed Sci. 1998 Sep;55(3):192-8.
Comparative study of oxidatively modified low-density lipoprotein (Ox-LDL) and malondialdehyde-modified low-density lipoprotein (MDA-LDL) is important for further understanding the biological properties of Ox-LDL, such as its toxic effects, immunogenicity and multiplicity of scavenger receptor binding. In this study, the characteristics of Ox-LDL and MDA-LDL binding and degradation were compared. The results show that when their degree of modification (as determined by relative electrophoretic mobility) was similar, the binding and degradation of Ox-LDL by the macrophage cell line P388D1 were greater than those of MDA-LDL. The binding and degradation of Ox-LDL by macrophages and human umbilical vein endothelial cells increased with the degree of modification. In addition, Ox-LDL or MDA-LDL could competitively inhibit binding of labelled Ox-LDL or labelled MDA-LDL to their respective macrophage receptors, and could partially inhibit each other.
氧化修饰低密度脂蛋白（Ox-LDL）和丙二醛修饰低密度脂蛋白（MDA-LDL）的比较研究对于进一步了解Ox-LDL的生物学特性非常重要，例如其毒性作用、免疫原性和清道夫受体结合的多样性。在本研究中，比较了Ox-LDL和MDA-LDL的结合及降解特性。结果表明，当它们的修饰程度（由相对电泳迁移率确定）相似时，巨噬细胞系P388D1对Ox-LDL的结合和降解大于对MDA-LDL的结合和降解。巨噬细胞和人脐静脉内皮细胞对Ox-LDL的结合和降解随修饰程度增加。此外，Ox-LDL或MDA-LDL可竞争性抑制标记的Ox-LDL或标记的MDA-LDL与其各自巨噬细胞受体的结合，并且可部分相互抑制。
