Wang G, Deng Z, Qu Z
Department of Pathology, Tongji Medical University, Wuhan.
Zhonghua Yi Xue Za Zhi. 1997 Mar;77(3):212-5.
To examine whether oxidatively modified low density lipoprotein (OX-VLDL) and very low density lipoprotein (OX-VLDL) induce the expression of MCP-1 mRNA and protein by rabbit peritoneal exudate macrophages, and to clarify what a role of both lipoproteins play in atherogenesis.
After exposure of the macrophages to 25 micrograms/ml of LDL, VLDL, OX-LDL and OX-VLDL respectively, and a 24 hour incubation at 37 degrees C, the total RNA was extracted from the cells by guanidinium isothiocyanate method, and the media conditioned by the cultured macrophages were collected. Meanwhile, MCP-1 protein in the conditioned media was determined by using sandwich ELISA. Monocyte migration induced by the media was assayed by micropore filter method using modified Boyden chamber.
After a 24 hour exposure to OX-LDL and OX-VLDL, the MCP-1 mRNA expression in macrophages was markedly increased (3.2-fold and 3.4-fold, respectively), and the level of MCP-1 protein was also increased (2.2-fold and 2.5-fold, separately), and furthermore, the monocyte migration distance was significantly increased. However, the expression of MCP-1 mRNA and protein was only slightly increased when exposed to LDL or VLDL.
Rabbit peritoneal exudate macrophages can express MCP-1 mRNA and protein, and OX-LDL and OX-VLDL induce stronger MCP-1 mRNA and protein expression in the cells.
研究氧化修饰的极低密度脂蛋白（OX-VLDL）和极低密度脂蛋白（VLDL）是否能诱导兔腹膜渗出巨噬细胞表达单核细胞趋化蛋白-1（MCP-1）的mRNA和蛋白，并阐明这两种脂蛋白在动脉粥样硬化形成中所起的作用。
将巨噬细胞分别暴露于25微克/毫升的低密度脂蛋白（LDL）、极低密度脂蛋白（VLDL）、氧化低密度脂蛋白（OX-LDL）和氧化极低密度脂蛋白（OX-VLDL）中，于37℃孵育24小时后，采用异硫氰酸胍法从细胞中提取总RNA，并收集经培养巨噬细胞处理过的培养基。同时，采用夹心酶联免疫吸附测定法（ELISA）检测处理过的培养基中的MCP-1蛋白。采用改良的Boyden小室微孔滤膜法检测培养基诱导的单核细胞迁移。
暴露于OX-LDL和OX-VLDL 24小时后，巨噬细胞中MCP-1的mRNA表达显著增加（分别为3.2倍和3.4倍），MCP-1蛋白水平也升高（分别为2.2倍和2.5倍），此外，单核细胞迁移距离显著增加。然而，暴露于LDL或VLDL时，MCP-1的mRNA和蛋白表达仅略有增加。
兔腹膜渗出巨噬细胞可表达MCP-1的mRNA和蛋白，OX-LDL和OX-VLDL可诱导细胞中更强的MCP-1 mRNA和蛋白表达。
