Kumagai N, Uchikoshi T
Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, Kawasaki, Japan.
Ann Plast Surg. 1997 Jul;39(1):68-73. doi: 10.1097/00000637-199707000-00012.
Successful repigmentation was achieved in 6 patients with three types of hypomelanosis (vitiligo, piebaldism, and albinism) by transplantation of fresh, autologous cultured epithelium with melanocytes. A small piece of uninvolved skin was taken for cultivation from a site adjacent to the lesion. Epidermal cells were cultured according to Green's technique. The lesions were abraded superficially and autologous cultured epithelium was applied. The grafts with functional melanocytes took completely and the wounds healed with minimal scarring. Repigmentation was visible within 6 to 8 months. The skin color resembled the surrounding normal skin except in the albinistic patient, in whom the donor skin was taken from a hyperpigmented area. Histochemical examination revealed dopa-positive melanocytes 12 to 17 days after grafting in the basal layer of the epidermis and the dermis. These cells grew in the basal layer of the epidermis and the hair follicles. Melanistic granules were visible in the keratinocytes in 1.5 months. A normal number of dopa-positive melanocytes and melanistic granules were seen in approximately 8 months. Thus, the autologous cultured epithelial grafting procedure is a promising treatment for patients with hypomelanosis.
通过移植带有黑素细胞的新鲜自体培养上皮，6例患有三种类型色素减退症（白癜风、斑驳病和白化病）的患者实现了成功的色素再生。从病变附近部位取一小片未受累皮肤进行培养。表皮细胞按照格林技术进行培养。对病变进行浅表皮磨除，然后应用自体培养上皮。带有功能性黑素细胞的移植物完全存活，伤口愈合且瘢痕形成最小。色素再生在6至8个月内可见。除白化病患者外，皮肤颜色与周围正常皮肤相似，白化病患者的供皮取自色素沉着过度区域。组织化学检查显示，移植后12至17天，表皮和真皮基底层有多巴阳性黑素细胞。这些细胞在表皮基底层和毛囊中生长。1.5个月时，角质形成细胞中可见黑素颗粒。大约8个月时可见正常数量的多巴阳性黑素细胞和黑素颗粒。因此，自体培养上皮移植手术对于色素减退症患者是一种有前景的治疗方法。
