Listrat A, Picard B, Geay Y
Laboratoire Croissance et Métabolismes des Herbivores, INRA, Saint-Genès-Champanelle, France.
Tissue Cell. 1999 Feb;31(1):17-27. doi: 10.1054/tice.1998.0015.
The aim of this work was to study the variability in the quantities of hydroxyproline, of type I and III collagens and in the location of types, I, III, IV, V, VI in four muscles of normal and double-muscled (DM) cattle. Samples were collected from foetuses at different ages post-conception. Both in the two genetic types and in muscles, from 110 days, types I, III, V, VI were located in perymisium and types I, IV, V, VI in endomysium. The amounts of hydroxyproline and of type I collagen increased from 150 to 180 or 230 days then decreased up to 260 days, with a trend to lower quantities in muscles of DM animals. Depending on the muscle and of the genetic type, amounts of type III, or changed as those of type I, or remained stable. Whatever the genetic type, at the end of gestation, proportions of type I and III in the total collagen are not identical in the four muscles, differences between muscles being particularly marked for type III, CT and MA muscles being the richer in this type. In addition, these two muscles contained less type III in DM animals than in normal ones.
本研究旨在探讨正常牛和双肌牛（DM）的四块肌肉中羟脯氨酸、I型和III型胶原蛋白的含量变化以及I、III、IV、V、VI型胶原蛋白的分布位置。从不同受孕后天数的胎儿中采集样本。在两种遗传类型的牛以及各肌肉中，从受孕110天起，I、III、V、VI型胶原蛋白位于肌束膜，I、IV、V、VI型胶原蛋白位于肌内膜。羟脯氨酸和I型胶原蛋白的含量从150天增加到180天或230天，然后在260天前下降，且DM牛肌肉中的含量有降低趋势。根据肌肉和遗传类型的不同，III型胶原蛋白的含量变化与I型相同或保持稳定。无论遗传类型如何，在妊娠末期，四块肌肉中I型和III型胶原蛋白在总胶原蛋白中的比例并不相同，肌肉间差异在III型胶原蛋白上尤为明显，CT肌和MA肌中该型胶原蛋白含量更丰富。此外，这两块肌肉中，DM牛的III型胶原蛋白含量低于正常牛。
