te Kronnie G, Stroband H, Schipper H, Samallo J
Wageningen Institute of Animal Sciences, Experimental Zoology Group, Wageningen Agricultural University, Marijkeweg 40, 6709 PG Wageningen, The Netherlands.
Dev Genes Evol. 1999 Jul;209(7):443-6. doi: 10.1007/s004270050276.
The Zfcth1 gene is, as the previously cloned carp cth1 gene, related to the mammalian TIS 11 family of primary response genes and encodes a protein with two putative CCCH zinc fingers. This report describes the RNA expression of this gene during oogenesis and early embryogenesis up to gastrulation in the zebrafish (Danio rerio). Maternal cth1 message is present in the ovary of 1-month-old fish and of adult fish in oocytes at all stages of maturation. In the youngest oocytes the message is localized in the cytoplasm all around the nucleus, in larger oocytes the message becomes restricted to the future animal pole of the embryo, and in mature oocytes the expression is sharply localized in the cortical layer under the micropyle. After ovulation the cth1 messenger spreads over the cytoplasmic cap and is distributed over the blastomeres during subsequent cleavages. In subsequent stages maternal expression of cth1 gradually disappears. From early epiboly stages onward embryonic cth1 expression is localized to the germ ring and the hypoblast cells in the central part of the embryonic shield. In the shield, cth1 expression largely overlaps with the area of gooscoid expression in the first involuting cells. In stages after 70% of epiboly cth1 expression diminishes and soon can no longer be detected in the embryo. Next to a developmental role in cell fate determination we propose a function for cth1 during oocyte maturation.
与先前克隆的鲤鱼cth1基因一样，Zfcth1基因与哺乳动物初级反应基因的TIS 11家族相关，并编码一种具有两个推定的CCCH锌指的蛋白质。本报告描述了该基因在斑马鱼（Danio rerio）卵子发生和直至原肠胚形成的早期胚胎发育过程中的RNA表达情况。母源性cth1信息存在于1月龄鱼的卵巢以及成年鱼处于所有成熟阶段的卵母细胞中。在最年幼的卵母细胞中，该信息定位于细胞核周围的细胞质中，在较大的卵母细胞中，该信息局限于胚胎未来的动物极，而在成熟卵母细胞中，表达则急剧定位于卵孔下方的皮层。排卵后，cth1信使扩散到细胞质帽上，并在随后的卵裂过程中分布于卵裂球上。在随后的阶段，cth1的母源表达逐渐消失。从早期外包阶段开始，胚胎cth1表达定位于胚环和胚胎盾中央部分的下胚层细胞。在胚胎盾中，cth1表达在很大程度上与第一批内卷细胞中gooscoid表达的区域重叠。在70%外包阶段之后的阶段，cth1表达减弱，很快在胚胎中就检测不到了。除了在细胞命运决定中的发育作用外，我们还提出cth1在卵母细胞成熟过程中具有功能。
