Koide N, Watanabe H, Yazawa K, Adachi W, Amano J
Second Department of Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
Hepatogastroenterology. 1999 Mar-Apr;46(26):944-51.
BACKGROUND/AIMS: Thymidine phosphorylase (dThdPase) is identical to platelet-derived endothelial cell growth factor which is an angiogenic factor. We attempted to clarify the significance of dThdPase expression in esophageal squamous cell carcinoma (SCC).
Tissues samples were taken from 50 patients with esophageal SCC after curative surgery. The expression of dThdPase was immunohistochemically examined using a monoclonal antibody to dThdPase (clone 654-1). Microvessels in SCC stained for Factor VIII-related antigen were counted. Vascular endothelial growth factor (VEGF) was immunostained with R11. Ki-67 antigen was immunostained with MIB-1, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end labeling was performed, and Ki-67 labeling index (LI) and apoptotic index were calculated.
The expression of dThdPase was observed in 30 patients (60%). Between the SCC with and without dThdPase expression, significant differences were found in microvessel count (p < 0.001) and VEGF expression (p < 0.01), but not in Ki-67 LI and apoptotic index. With regard to the clinicopathologic factors, significant differences were observed in histologic venous invasion (p < 0.01) and lymph node metastasis (p < 0.05). Survival rate after surgery was better in the patients with dThdPase-negative SCC (p < 0.05), and distant organ metastasis after surgery was frequently observed in the patients with dThdPase-positive SCC (p < 0.05).
These results suggest that dThdPase expression may be associated with angiogenic promotion and may be one of the prognostic factors in esophageal SCC.
背景/目的：胸苷磷酸化酶（dThdPase）与血小板衍生的内皮细胞生长因子相同，后者是一种血管生成因子。我们试图阐明dThdPase表达在食管鳞状细胞癌（SCC）中的意义。
对50例接受根治性手术的食管SCC患者采集组织样本。使用抗dThdPase单克隆抗体（克隆654-1）通过免疫组织化学方法检测dThdPase的表达。对SCC中与VIII因子相关抗原染色的微血管进行计数。用R11对血管内皮生长因子（VEGF）进行免疫染色。用MIB-1对Ki-67抗原进行免疫染色，进行末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸生物素缺口末端标记，并计算Ki-67标记指数（LI）和凋亡指数。
在30例患者（60%）中观察到dThdPase的表达。在有和没有dThdPase表达的SCC之间，微血管计数（p < 0.001）和VEGF表达（p < 0.01）存在显著差异，但在Ki-67 LI和凋亡指数方面没有差异。关于临床病理因素，在组织学静脉侵犯（p < 0.01）和淋巴结转移（p < 0.05）方面观察到显著差异。dThdPase阴性SCC患者的术后生存率较好（p < 0.05），dThdPase阳性SCC患者术后远处器官转移较为常见（p < 0.05）。
这些结果表明，dThdPase表达可能与血管生成促进有关，可能是食管SCC的预后因素之一。
