Knapik E W, Goodman A, Ekker M, Chevrette M, Delgado J, Neuhauss S, Shimoda N, Driever W, Fishman M C, Jacob H J
Cardiovascular Research Center, Massachusetts General Hospital, Charlestown 02129, USA.
Nat Genet. 1998 Apr;18(4):338-43. doi: 10.1038/ng0498-338.
We have constructed a zebrafish genetic linkage map consisting of 705 simple sequence-length polymorphism markers (SSLPs). The map covers 2350 centimorgans (cM) of the zebrafish genome with an average resolution of 3.3 cM. It is a complete map in genetic mapping terms (there is one linkage group for each of the 25 chromosomes), and it has been confirmed by somatic-cell hybrids and centromere-mapping using half-tetrad analysis. The markers are highly polymorphic in the zebrafish strains used for genetic crosses and provide a means to compare genetic segregation of developmental mutations between laboratories. These markers will provide an initial infrastructure for the positional cloning of the nearly 600 zebrafish genes identified as crucial to vertebrate development,and will become the anchor for the physical map of the zebrafish genome.
我们构建了一个由705个简单序列长度多态性标记（SSLPs）组成的斑马鱼遗传连锁图谱。该图谱覆盖了斑马鱼基因组的2350厘摩（cM），平均分辨率为3.3 cM。从遗传图谱的角度来看，它是一个完整的图谱（25条染色体各有一个连锁群），并且已经通过体细胞杂种和使用半四分体分析的着丝粒作图得到了证实。这些标记在用于遗传杂交的斑马鱼品系中具有高度多态性，并提供了一种方法来比较不同实验室之间发育突变的遗传分离情况。这些标记将为近600个被确定对脊椎动物发育至关重要的斑马鱼基因的定位克隆提供初步的基础设施，并将成为斑马鱼基因组物理图谱的锚定。
