Watanabe T K, Okuno S, Oga K, Mizoguchi-Miyakita A, Tsuji A, Yamasaki Y, Hishigaki H, Kanemoto N, Takagi T, Takahashi E, Irie Y, Nakamura Y, Tanigami A
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawauchi-cho, Tokushima, 771-0192, Japan.
Genomics. 1999 Jun 15;58(3):233-9. doi: 10.1006/geno.1999.5789.
To identify genetic determinants relevant to non-insulin-dependent diabetes mellitus (NIDDM), we performed a genome-wide analysis for quantitative trait loci (QTLs) using 359 backcross progeny of the Otsuka Long-Evans Tokushima Fatty (OLETF) rat. The OLETF strain is a well-studied animal model of obese NIDDM, with features of hyperinsulinemia, hyperglycemia, insulin resistance, and abundant abdominal fat. Our extensive genomic scanning with 218 markers revealed nine significant QTLs, including a strong determinant of obesity on chromosome 1 (Dmo1: LOD = 13.99, for body weight). Two highly significant QTLs for glucose homeostasis were found, one on chromosome 1 (Dmo4 LOD = 7.16, for postprandial glucose level) and the other on chromosome X (Dmo11/Odb1: LOD = 7.81, for postprandial glucose level). These data are comparable to results of our previous studies of the OLETF rat.
为了确定与非胰岛素依赖型糖尿病（NIDDM）相关的遗传决定因素，我们使用大冢长崎德岛肥胖（OLETF）大鼠的359个回交后代进行了全基因组数量性状基因座（QTL）分析。OLETF品系是一种经过充分研究的肥胖NIDDM动物模型，具有高胰岛素血症、高血糖、胰岛素抵抗和大量腹部脂肪等特征。我们用218个标记进行的广泛基因组扫描揭示了9个显著的QTL，包括1号染色体上肥胖的一个强决定因素（Dmo1：LOD = 13.99，针对体重）。发现了两个与葡萄糖稳态高度相关的QTL，一个在1号染色体上（Dmo4 LOD = 7.16，针对餐后血糖水平），另一个在X染色体上（Dmo11/Odb1：LOD = 7.81，针对餐后血糖水平）。这些数据与我们之前对OLETF大鼠的研究结果相当。
