Ogino T, Wei S, Wei K, Moralejo D H, Kose H, Mizuno A, Shima K, Sasaki Y, Yamada T, Matsumoto K
Department of Laboratory Medicine, Institute for Animal Experimentation, University of Tokushima School of Medicine, 3 Kuramoto, Tokushima, 770-8503, Japan.
Genomics. 2000 Nov 15;70(1):19-25. doi: 10.1006/geno.2000.6349.
The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is an animal model for obese type 2 diabetes in human. Obesity is essential for the onset of type 2 diabetes in this rat. Our present investigation was designed to identify quantitative trait loci (QTLs) contributing to obesity by performing a whole-genome search using 214 F(2) intercross progeny between OLETF and F344 rats. We have identified six QTLs responsible for adiposity indices of fat pads on rat chromosomes 2 (Obs1 for mesenteric fat), 4 (Obs2 for retroperitoneal fat), 8 (Obs3 for mesenteric fat), 9 (Obs4 for retroperitoneal fat), and 14 (Obs5 and Obs6 for retroperitoneal fat), demonstrating that the adiposity indices of individual fat pads were under the control of different genes. As expected, the OLETF allele corresponds to increased adiposity indices for all QTLs, except for Obs3, in which the F344 allele leads to an increase in the index.
大冢长-艾氏德岛肥胖（OLETF）大鼠是人类肥胖型2型糖尿病的动物模型。肥胖是该大鼠发生2型糖尿病的必要条件。我们目前的研究旨在通过使用214只OLETF大鼠与F344大鼠杂交产生的F(2)代杂交后代进行全基因组搜索，来鉴定导致肥胖的数量性状基因座（QTL）。我们已经在大鼠染色体2（肠系膜脂肪的Obs1）、4（腹膜后脂肪的Obs2）、8（肠系膜脂肪的Obs3）、9（腹膜后脂肪的Obs4）和14（腹膜后脂肪的Obs5和Obs6）上鉴定出6个负责脂肪垫肥胖指数的QTL，表明各个脂肪垫的肥胖指数受不同基因的控制。正如预期的那样，除了Obs3外，所有QTL的OLETF等位基因都对应于肥胖指数的增加，在Obs3中，F344等位基因导致指数增加。
