Yanabe M, Shibuya M, Gonda T, Asai H, Tanaka T, Narita T, Sudo K, Itoh K
Japan SLC, Inc., Shizuoka, Japan.
Exp Anim. 1999 Apr;48(2):101-6. doi: 10.1538/expanim.48.101.
In the present study we attempted to establish specific-pathogen-free (SPF) rabbit breeding colonies with two groups of limited-flora (LF) rabbits, both ex-germfree rabbits, and their offspring. Two groups of LF rabbits associated with cecal flora of conventional (CV) rabbits produced in a previous study [Exp. Animals, submitted], were transferred to individual barrier rooms and some of the LF rabbits were accommodated in isolators to maintain the basic flora for SPF rabbits. The composition of the cecal flora of LF rabbits was stable for a long period; bacteroides remained predominant and clostridia dominant. From the SPF rabbits, different types of bacteria, e.g., enterobacteriaceae and streptococci, which could not be isolated in the isolator were detected at a low population level at an early stage in the establishment of the SPF colonies, but the basic composition of the cecal flora was mainly bacteroidaceae and clostridia and did not change over a long period, and the floral composition became similar to that of CV rabbits. The fertility and weaning rates of the SPF rabbits were satisfactory for a SPF rabbit colony. In addition, these SPF colonies were free of more than one year rabbit-specific pathogens.
在本研究中，我们试图用两组有限菌群（LF）兔建立无特定病原体（SPF）兔繁殖群体，这两组LF兔均为无菌兔及其后代。将先前研究[《实验动物》，待发表]中产生的与常规（CV）兔盲肠菌群相关的两组LF兔转移到单独的屏障室，部分LF兔安置在隔离器中以维持SPF兔的基本菌群。LF兔盲肠菌群的组成长期稳定；拟杆菌仍占主导，梭菌为优势菌。在建立SPF群体的早期阶段，从SPF兔中检测到了在隔离器中无法分离的不同类型细菌，如肠杆菌科和链球菌，但数量较低，且盲肠菌群的基本组成主要是拟杆菌科和梭菌，长期未发生变化，菌群组成变得与CV兔相似。SPF兔的繁殖力和断奶率对于一个SPF兔群体来说是令人满意的。此外，这些SPF群体超过一年未感染兔特异性病原体。
