Kuang Y, Hou C, Gou S
Department of Orthopedics Surgery, Changzheng Hospital, 2nd Military Medical University, Shanghai, P. R. China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 1998 Mar;12(2):90-3.
In order to study the effect of chitin and chitosan on the growth of Schwann cell (SC) of rats in vitro, the SC was isolated from sciatic nerve and brachial plexus of new-born rats. After the enzymatic and mechanical dissociation, the cell suspension was vaccinated on chitin membrane and chitosan fluid-coated glass coverslips. Then, the growth of SC was examined at 1, 3, 7 days after culture under light microscope and scanning electron microscope. The results showed that 94 percent of the cell grown from was SC and only 6% was fibroblast (FB), while that of the control SC 71% and FB 29% in population. The number of SC in chitosan suspension was more than that in chitin. Therefore, the conclusion was that the chitin and chitosan was histocompatible to SC, and chitosan suspension was superior to chitin, and both could inhibit the growth of fibroblast.
为研究几丁质和壳聚糖对大鼠雪旺细胞（SC）体外生长的影响，从新生大鼠坐骨神经和臂丛神经中分离出雪旺细胞。经酶解和机械解离后，将细胞悬液接种于几丁质膜和壳聚糖溶液包被的盖玻片上。然后，在培养1、3、7天后，于光学显微镜和扫描电子显微镜下观察雪旺细胞的生长情况。结果显示，培养所得细胞中94%为雪旺细胞，仅6%为成纤维细胞（FB），而对照雪旺细胞群体中雪旺细胞占71%，成纤维细胞占29%。壳聚糖悬液中的雪旺细胞数量多于几丁质中的。因此，得出结论：几丁质和壳聚糖与雪旺细胞具有组织相容性，壳聚糖悬液优于几丁质，且二者均能抑制成纤维细胞生长。
