持久性病毒整合到其藻类宿主——硅藻（褐藻门）的基因组中。

Persistent virus integration into the genome of its algal host, Ectocarpus siliculosus (Phaeophyceae).

作者信息

Delaroque N, Maier I, Knippers R, M ºller D G

出版信息

J Gen Virol. 1999 Jun;80 ( Pt 6):1367-1370. doi: 10.1099/0022-1317-80-6-1367.

Abstract

The brown alga Ectocarpus siliculosus frequently carries an endogenous virus, E. siliculosus virus (EsV-1), the genome of which is a circular, double-stranded DNA molecule of about 320 kbp. After infection, which occurs in the unicellular spores or gametes, the virus is present latently in all somatic cells of the host. Virus multiplication is restricted to cells of the reproductive organs. It has been an open question whether the latent viral DNA occurs as a free episome or becomes integrated into the host genome. PCR studies showed that viral DNA co-migrates with high molecular mass DNA in pulsed-field gel electrophoresis, which confirms that latent viral DNA is integrated into the host genome.

摘要

褐藻硅藻经常携带一种内源性病毒，即硅藻病毒（EsV-1），其基因组是一个约320千碱基对的环状双链DNA分子。感染发生在单细胞孢子或配子中，病毒在宿主的所有体细胞中潜伏存在。病毒增殖仅限于生殖器官的细胞。潜伏的病毒DNA是以游离附加体形式存在还是整合到宿主基因组中，一直是个悬而未决的问题。聚合酶链反应（PCR）研究表明，在脉冲场凝胶电泳中，病毒DNA与高分子量DNA共同迁移，这证实了潜伏的病毒DNA已整合到宿主基因组中。