Ivey R G, Henry E C, Lee A M, Klepper L, Krueger S K, Meints R H
Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331-2906, USA.
Virology. 1996 Jun 15;220(2):267-73. doi: 10.1006/viro.1996.0314.
Persistent viruses occur intracellularly in brown algae, specifically the Ectocarpales, and as reported here in the genus Feldmannia. Feldmannia species are small (1 mm-several cm), filamentous forms with single-celled meiotic sporangia that normally produce haploid zoospores. In the isolate reported here, spores were not observed in the sporangia but rather numerous (approximately 10(6) per cell) polyhedral viruses are formed in their place. Two dsDNA genome classes of 158 and 178 kbp, with two restriction site variants of each, are described. The individual abundance of each genome in viral preparations is affected by culture temperature. A cosmid library was used to generate circular restriction enzyme (BamHi, Noti, and Psti) site maps.
持久性病毒存在于褐藻细胞内，特别是在网地藻目，以及本文所报道的费尔德曼藻属中。费尔德曼藻属物种体型较小（1毫米至几厘米），为丝状形态，具有单细胞减数分裂孢子囊，通常产生单倍体游动孢子。在本文报道的分离物中，孢子囊中未观察到孢子，而是在其位置形成了大量（每个细胞约10⁶个）多面体病毒。描述了158和178千碱基对的两个双链DNA基因组类别，每个类别有两个限制性酶切位点变体。病毒制剂中每个基因组的个体丰度受培养温度影响。使用黏粒文库生成环状限制性内切酶（BamHi、Noti和Psti）位点图谱。
