Lee A M, Ivey R G, Meints R H
Center for Gene Research and Biotechnology, Oregon State University, Corvallis, Oregon, 97331-2906, USA.
Virology. 1998 Aug 15;248(1):35-45. doi: 10.1006/viro.1998.9245.
We describe the structure of a Feldmannia sp. virus (FsV) genome integrated in the brown alga, Feldmannia. This integrated FsV genome appears to be permanently inactivated and lost its ability to excise and replicate. Unlike the replicated form of FsV, this integrated FsV genome contains a large (>50 kb) repeat region inserted in a protein kinase open reading frame. While related to the 173-bp repeats previously characterized in the FsV genome (Lee et al., 1995), Southern blot analysis indicates that the repeats in the inactive, integrated FsV genome are distinct from those previously characterized. Fine structural analysis of the repeat-insertion sites in the protein kinase gene indicates that there are 8- and 10-bp palindromic sequences present in multiple locations located near the repeat-insertion site. The translated protein kinase contains all of the catalytic motifs conserved in most serine/threonine protein kinases and a potential autophosphorylation site. This protein kinase gene is expressed as RNA in sporophyte plants where virus production is active but not in gametophyte plants where the virus genome is latent. The structure of the integrated virus genome is discussed.
我们描述了整合在褐藻费尔德曼藻（Feldmannia）中的费尔德曼藻病毒（FsV）基因组的结构。这种整合的FsV基因组似乎已永久失活，失去了切除和复制的能力。与复制形式的FsV不同，这种整合的FsV基因组在蛋白激酶开放阅读框中插入了一个大的（>50 kb）重复区域。虽然与先前在FsV基因组中鉴定的173 bp重复序列相关（Lee等人，1995年），但Southern印迹分析表明，失活的整合FsV基因组中的重复序列与先前鉴定的不同。对蛋白激酶基因中重复插入位点的精细结构分析表明，在靠近重复插入位点的多个位置存在8 bp和10 bp的回文序列。翻译后的蛋白激酶包含大多数丝氨酸/苏氨酸蛋白激酶中保守的所有催化基序和一个潜在的自磷酸化位点。该蛋白激酶基因在病毒产生活跃的孢子体植物中以RNA形式表达，但在病毒基因组潜伏的配子体植物中不表达。我们讨论了整合病毒基因组的结构。
