Holtbäck U, Brismar H, DiBona G F, Fu M, Greengard P, Aperia A
Department of Women and Children's Health, Karolinska Institutet, Astrid Lindgren's Children's Hospital, Q2:09, 171 76 Stockholm, Sweden.
Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7271-5. doi: 10.1073/pnas.96.13.7271.
There is a great deal of evidence for synergistic interactions between G protein-coupled signal transduction pathways in various tissues. As two specific examples, the potent effects of the biogenic amines norepinephrine and dopamine on sodium transporters and natriuresis can be modulated by neuropeptide Y and atrial natriuretic peptide, respectively. Here, we report, using a renal epithelial cell line, that both types of modulation involve recruitment of receptors from the interior of the cell to the plasma membrane. The results indicate that recruitment of G protein-coupled receptors may be a ubiquitous mechanism for receptor sensitization and may play a role in the modulation of signal transduction comparable to that of the well established phenomenon of receptor endocytosis and desensitization.
有大量证据表明,各种组织中的G蛋白偶联信号转导途径之间存在协同相互作用。作为两个具体例子,生物胺去甲肾上腺素和多巴胺对钠转运体和利钠作用的强效影响可分别被神经肽Y和心房利钠肽调节。在此,我们使用一种肾上皮细胞系报告,这两种调节类型均涉及细胞内受体向质膜的募集。结果表明,G蛋白偶联受体的募集可能是受体致敏的普遍机制,并且可能在信号转导调节中发挥作用,其作用类似于已确立的受体内吞和脱敏现象。