菊苣对肥大细胞介导的速发型过敏反应的抑制作用。
Inhibitory effect of mast cell-mediated immediate-type allergic reactions by Cichorium intybus.
作者信息
Kim H M, Kim H W, Lyu Y S, Won J H, Kim D K, Lee Y M, Morii E, Jippo T, Kitamura Y, An N H
机构信息
College of Pharmacy, Wonkwang University, Iksan, Chonbuk, 570-749, South Korea.
出版信息
Pharmacol Res. 1999 Jul;40(1):61-5. doi: 10.1006/phrs.1999.0474.
We investigated the effect of an aqueous extract of Cichorium intybus (CIAE) on mast cell-mediated immediate type allergic reactions. CIAE (0.1-1000 mg kg-1) dose-dependently inhibited systemic anaphylactic reaction induced by compound 48/80 in mice. Especially, CIAE inhibited compound 48/80-induced anaphylactic reaction 100% with the dose of 1000 mg kg-1. CIAE 1000 mg kg-1also significantly inhibited local anaphylactic reaction activated by anti-dinitrophenyl (DNP) IgE. When mice were pretreated with CIAE at a concentration ranging from 0.1 to 1000 mg kg-1, the plasma histamine levels were reduced in a dose-dependent manner. CIAE (1-1000 microg ml-1) dose-dependently inhibited histamine release from the rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. The level of cAMP in RPMC, when CIAE (1000 microg ml-1) was added, increased significantly compared with that of control cells. These results indicate that CIAE inhibits mast cell-mediated immediate-type allergic reactions in vivo and in vitro.
我们研究了菊苣水提取物（CIAE）对肥大细胞介导的速发型过敏反应的影响。CIAE（0.1 - 1000 mg kg-1）剂量依赖性地抑制了化合物48/80在小鼠中诱导的全身过敏反应。特别是，CIAE在1000 mg kg-1剂量时100%抑制了化合物48/80诱导的过敏反应。CIAE 1000 mg kg-1也显著抑制了抗二硝基苯基（DNP）IgE激活的局部过敏反应。当用浓度范围为0.1至1000 mg kg-1的CIAE预处理小鼠时，血浆组胺水平呈剂量依赖性降低。CIAE（1 - 1000 μg ml-1）剂量依赖性地抑制了由化合物48/80或抗DNP IgE激活的大鼠腹膜肥大细胞（RPMC）释放组胺。当加入CIAE（1000 μg ml-1）时，RPMC中的cAMP水平与对照细胞相比显著增加。这些结果表明，CIAE在体内和体外均抑制肥大细胞介导的速发型过敏反应。
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