Chalisova N I, Zhuravskiĭ S G, Penniiaĭnen V A, Berezhnoĭ S N, Artamonova I I, Zavalova L L, Baskova I P
Institute of Physiology, Russian Academy of Sciences, and Medical Institute, St. Petersburg.
Tsitologiia. 1999;41(1):48-52.
The effect of destabilase, a component of Hirudo medicinalis salivary gland secret, was investigated in organotypic tissue culture of dorsal root ganglia (DRG) of 10-11-day old chick embryos. Native destabilase in concentrations 0.01 and 0.05 ng/ml was active, inducing a more intensive neurite growth in DRG that in the control. The stabilizing activity of destabilase was lost following reverse-phase chromatography. Neurite-stimulating effects of the drug "pyjavit" is due presumably to neurite-stimulating activity of destabilase.
研究了欧洲医蛭唾液腺分泌物成分去稳定酶对10-11日龄鸡胚背根神经节（DRG）器官型组织培养的影响。浓度为0.01和0.05 ng/ml的天然去稳定酶具有活性，与对照组相比，可诱导DRG中神经突生长更为密集。经反相色谱法处理后，去稳定酶的稳定活性丧失。药物“皮贾维特”的神经突刺激作用可能归因于去稳定酶的神经突刺激活性。
