Tingsgaard P K, Bock T, Larsen P L, Tos M
Department of Oto-rhino-laryngology, Head and Neck Surgery, Gentofte Hospital, University Hospital of Copenhagen, Denmark.
Acta Otolaryngol. 1999;119(3):362-8. doi: 10.1080/00016489950181404.
Infiltration of eosinophil granulocytes and endothelial expression of vascular cell adhesion molecule-1 (VCAM-1) and P-selectin was investigated in biopsies of polyps and inferior turbinates from 16 patients with nasal polyps, by the use of immunohistochemical staining and stereological quantification before, during and after topical treatment with budesonide (Rhinocort Turbuhaler). Before glucocorticoid treatment a higher density of eosinophil profiles (p < 0.005), making up a larger proportion of the total cellular infiltrate (p < 0.0005), was found in the polyps compared with the inferior turbinates. Endothelial VCAM-1 expression was higher in polyps than in inferior turbinates (p < 0.005), in contrast with the expression of P-selectin, which was more frequently expressed in the inferior turbinates (p < 0.05). Topical glucocorticoid treatment reduced the density of eosinophil profiles (p < 0.05) and the endothelial expression of VCAM-1 (p < 0.007) and P-selectin (p < 0.02) in polyps. Eosinophil counts and VCAM-1 expression returned to pre-treatment levels 8 weeks after discontinuation of budesonide treatment. The observed reduction in endothelial expression of cellular adhesion molecules may interfere with cellular recruitment in nasal polyps and thus contribute to the known anti-inflammatory effect of glucocorticoid in nasal polyposis.
通过免疫组织化学染色和体视学定量方法，对16例鼻息肉患者息肉和下鼻甲活检组织中嗜酸性粒细胞的浸润以及血管细胞黏附分子-1（VCAM-1）和P-选择素的内皮表达情况进行了研究，研究时间为布地奈德（雷诺考特都保）局部治疗前、治疗期间和治疗后。在糖皮质激素治疗前，与下鼻甲相比，息肉中嗜酸性粒细胞轮廓的密度更高（p<0.005），在总细胞浸润中所占比例更大（p<0.0005）。息肉中内皮VCAM-1的表达高于下鼻甲（p<0.005），而P-选择素的表达情况则相反，下鼻甲中P-选择素表达更频繁（p<0.05）。局部糖皮质激素治疗降低了息肉中嗜酸性粒细胞轮廓的密度（p<0.05）以及VCAM-1（p<0.007）和P-选择素（p<0.02）的内皮表达。停用布地奈德治疗8周后，嗜酸性粒细胞计数和VCAM-1表达恢复到治疗前水平。观察到的细胞黏附分子内皮表达的降低可能会干扰鼻息肉中的细胞募集，从而有助于糖皮质激素在鼻息肉病中已知的抗炎作用。
