Eweiss Ahmed, Dogheim Yakout, Hassab Mohammed, Tayel Hanan, Hammad Zaki
Department of Otolaryngology, Alexandria Faculty of Medicine, Alexandria, Egypt.
Eur Arch Otorhinolaryngol. 2009 Mar;266(3):377-83. doi: 10.1007/s00405-008-0762-1. Epub 2008 Jul 19.
The objective of our study was to investigate: the expression of vascular cell adhesion molecule-1 (VCAM-1) in diffuse sino-nasal polyps; the relation between VCAM-1 and selective eosinophilia in diffuse sino-nasal polyps; the effect of eosinophilia and VCAM-1 expression on recurrence of diffuse sino-nasal polyps following treatment. Fifty patients with diffuse sino-nasal polyps were included in the study. Biopsies were taken from the polyps and the inferior turbinate mucosa of each patient and were studied by immunohistochemical staining for detection of VCAM-1 expression on the blood vessels, as well as by hematoxylin and eosin (H&E) staining for detection of eosinophils. The percentage of blood vessels expressing VCAM-1 and the number of eosinophils in the polyps were detected and correlated and then compared to the corresponding numbers in the inferior turbinates. All patients were treated by endoscopic ethmoidectomies followed by local steroids for 1 year. Recurrence of polyps was monitored during the post-operative follow-up period. The quantitative expression of VCAM-1 and number of infiltrating eosinophils were compared in patients who developed recurrent polyps and those who did not. VCAM-1 was expressed in the polyps but not significantly higher than in the inferior turbinates of the patients or the controls. Eosinophils were significantly higher in the polyps compared to the inferior turbinates. No correlation was found between the expression of the VCAM-1 and the number of eosinophils. VCAM-1 expression was significantly higher in patients developing recurrent polyps. No significant difference was found in the number of infiltrating eosinophils between patients developing and those not developing recurrent polyps. VCAM-1 is expressed in polyps but not significantly higher than its expression in the inferior turbinate mucosa. VCAM-1 expression in diffuse sino-nasal polyps cannot on its own explain the selective eosinophilia detected in this disease. High VCAM-1 expression in diffuse sino-nasal polyps may be an indicator of a higher probability of polyps recurrence. Degree of eosinophilic infiltration cannot be taken as such an indicator.
血管细胞黏附分子-1（VCAM-1）在弥漫性鼻息肉中的表达；VCAM-1与弥漫性鼻息肉中选择性嗜酸性粒细胞增多之间的关系；嗜酸性粒细胞增多和VCAM-1表达对弥漫性鼻息肉治疗后复发的影响。50例弥漫性鼻息肉患者纳入本研究。从每位患者的息肉和下鼻甲黏膜取活检组织，通过免疫组织化学染色研究血管上VCAM-1的表达，以及通过苏木精和伊红（H&E）染色检测嗜酸性粒细胞。检测并关联息肉中表达VCAM-1的血管百分比和嗜酸性粒细胞数量，然后与下鼻甲中的相应数量进行比较。所有患者均接受鼻内镜筛窦切除术，随后局部使用类固醇药物1年。术后随访期间监测息肉复发情况。比较息肉复发患者和未复发患者中VCAM-1的定量表达和浸润嗜酸性粒细胞数量。VCAM-1在息肉中表达，但并不显著高于患者或对照组的下鼻甲。与下鼻甲相比，息肉中的嗜酸性粒细胞显著增多。未发现VCAM-1表达与嗜酸性粒细胞数量之间存在相关性。息肉复发患者中VCAM-1表达显著更高。息肉复发患者和未复发患者之间浸润嗜酸性粒细胞数量未发现显著差异。VCAM-1在息肉中表达，但并不显著高于其在下鼻甲黏膜中的表达。弥漫性鼻息肉中VCAM-1的表达本身无法解释该疾病中检测到的选择性嗜酸性粒细胞增多。弥漫性鼻息肉中高VCAM-1表达可能是息肉复发可能性较高的一个指标。嗜酸性粒细胞浸润程度不能作为这样一个指标。
