Kodama H, Faden H, Harabuchi Y, Kataura A, Bernstein J M, Brodsky L
Department of Otolaryngology, Sapporo Medical University, Japan.
Acta Otolaryngol. 1999;119(3):377-83. doi: 10.1080/00016489950181422.
Cellular immune responses to the P6 outer membrane protein of non-typeable Haemophilus influenzae (NTHi) were determined in vitro by measuring immunoglobulin (Ig) secreting cells and lymphocyte proliferation in adenoidal and tonsillar lymphocytes from 19 children. Preliminary tests showed that P6 did not stimulate naive cells such as cord blood lymphocytes, but did stimulate sensitized cells in adenoids and tonsils. Cellular proliferation was significantly higher in adenoidal lymphocytes than in tonsillar lymphocytes (median: quadratile of stimulation index = 3.7:2.3-5.5 vs. 1.2:1.0-2.1, p < 0.02). A comparison between children with or without otitis media revealed that proliferative responses to P6 of adenoidal lymphocytes from children with otitis media were significantly decreased (2.0:1.8-3.6 vs. 3.7:2.3-5.5, p < 0.04). P6-specific antibody secreting cells were identified in a total of 14 adenoids and the number of cells secreting IgA was decreased in the otitis media group compared to controls (median: quadratile/10(6) cells = 435:359-499 vs. 755:593-1870, p < 0.05). Cultivation with P6 stimulated IgA secretion in children without otitis media, while no response was seen in children with otitis media (median: quadratile/10(6) cells = 1323:915-2410 vs. 2240:1900-2830, p < 0.02). These preliminary data demonstrate that lymphocytes from adenoids and tonsils recognize P6 as a specific antigen and that the adenoid is the more reactive of the two organs. Impaired P6-specific cellular immune responses of adenoids in children with otitis media may explain the recurrent nature of otitis media due to NTHi in the otitis prone population.
通过检测19名儿童腺样体和扁桃体淋巴细胞中免疫球蛋白（Ig）分泌细胞及淋巴细胞增殖情况，在体外测定了对不可分型流感嗜血杆菌（NTHi）P6外膜蛋白的细胞免疫反应。初步试验表明，P6不能刺激如脐血淋巴细胞等幼稚细胞，但能刺激腺样体和扁桃体中的致敏细胞。腺样体淋巴细胞中的细胞增殖显著高于扁桃体淋巴细胞（中位数：刺激指数四分位数 = 3.7:2.3 - 5.5 对比 1.2:1.0 - 2.1，p < 0.02）。对有或无中耳炎儿童的比较显示，中耳炎患儿腺样体淋巴细胞对P6的增殖反应显著降低（2.0:1.8 - 3.6 对比 3.7:2.3 - 5.5，p < 0.04）。在总共14个腺样体中鉴定出了P6特异性抗体分泌细胞，与对照组相比，中耳炎组分泌IgA的细胞数量减少（中位数：四分位数/10⁶细胞 = 435:359 - 499 对比 755:593 - 1870，p < 0.05）。用P6培养可刺激无中耳炎儿童的IgA分泌，而中耳炎儿童无反应（中位数：四分位数/10⁶细胞 = 1323:915 - 2410 对比 2240:1900 - 2830，p < 0.02）。这些初步数据表明，腺样体和扁桃体的淋巴细胞将P6识别为特异性抗原，且腺样体是这两个器官中反应性更强的。中耳炎患儿腺样体中P6特异性细胞免疫反应受损，可能解释了中耳炎易感人群中由NTHi引起的中耳炎的复发性。
