Harabuchi Y, Hamamoto M, Kodama H, Kataura A
Department of Otolaryngology, School of Medicine, Sapporo Medical University, Japan.
Int J Pediatr Otorhinolaryngol. 1996 Apr;35(2):117-25. doi: 10.1016/0165-5876(95)01298-2.
Spontaneous immunoglobulin (Ig) production by autologous adenoidal and tonsillar lymphocytes cultured without any mitogen was measured by an enzyme-linked immunosorbent assay (ELISA) in 18 children with or without otitis media with effusion (OME). Both IgG and IgA levels synthesized by adenoidal or tonsillar lymphocytes significantly increased with age. The average concentrations of IgG and IgA produced by adenoidal lymphocytes from children with OME were significantly lower than those from children without OME. Adenoidal lymphocytes produced IgG and IgM at significantly lower levels than autologous tonsillar lymphocytes did in children with OME. In contrast, no significant difference between adenoidal and tonsillar lymphocytes was seen on Ig production for any isotypes in children without OME. These results may indicate immunological impairment of the adenoids associated with OME.
通过酶联免疫吸附测定（ELISA），对18名有或无中耳积液（OME）的儿童中未添加任何促有丝分裂原培养的自体腺样体和扁桃体淋巴细胞的自发免疫球蛋白（Ig）产生情况进行了测量。腺样体或扁桃体淋巴细胞合成的IgG和IgA水平均随年龄显著增加。OME患儿腺样体淋巴细胞产生的IgG和IgA平均浓度显著低于无OME患儿。在OME患儿中，腺样体淋巴细胞产生IgG和IgM的水平显著低于自体扁桃体淋巴细胞。相比之下，在无OME患儿中，腺样体和扁桃体淋巴细胞在任何同种型的Ig产生上均未观察到显著差异。这些结果可能表明与OME相关的腺样体免疫功能受损。
