淋病奈瑟菌感染研究。十六。淋病奈瑟菌免疫球蛋白A1蛋白酶的纯化。
Studies on gonococcus infection. XVI. Purification of Neisseria gonorrhoeae immunoglobulin A1 protease.
作者信息
Blake M S, Swanson J
出版信息
Infect Immun. 1978 Nov;22(2):350-8. doi: 10.1128/iai.22.2.350-358.1978.
Abstract
A protease which cleaves human immunoglobulin A1 (IgA1) has been purified from broth cultures of Neisseria gonorrhoeae. This IgA1 protease is produced by pilated and nonpilated gonococci throughout their growth cycles. A combination of ammonium sulfate precipitation, column chromatography, and either isoelectric focusing or affinity chromatography was utilized to obtain an enzyme preparation that showed approximately 3,800-fold purification and exhibited two bands (65,000 and 70,000 daltons) by analytical polyacrylamide electrophoresis in the presence of sodium dodecyl sulfate and reducing conditions. IgA1 protease activity is dependent on divalent cations and is heat labile. Detection and quantitation of IgA protease activity utilized an assay in which [125I]IgA1 is incubated with protease preparations and the cleavage products are analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
摘要
一种可切割人免疫球蛋白A1（IgA1）的蛋白酶已从淋病奈瑟菌的肉汤培养物中纯化出来。这种IgA1蛋白酶由有菌毛和无菌毛的淋球菌在其整个生长周期中产生。通过硫酸铵沉淀、柱色谱以及等电聚焦或亲和色谱相结合的方法，获得了一种酶制剂，该制剂经分析型聚丙烯酰胺凝胶电泳（在十二烷基硫酸钠存在及还原条件下）显示出约3800倍的纯化效果，并呈现两条带（65000和70000道尔顿）。IgA1蛋白酶的活性依赖于二价阳离子，且对热不稳定。IgA蛋白酶活性的检测和定量采用一种检测方法，即将[125I]IgA1与蛋白酶制剂一起孵育，然后通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分析裂解产物。
相似文献
1
Studies on gonococcus infection. XVI. Purification of Neisseria gonorrhoeae immunoglobulin A1 protease.淋病奈瑟菌感染研究。十六。淋病奈瑟菌免疫球蛋白A1蛋白酶的纯化。
Infect Immun. 1978 Nov;22(2):350-8. doi: 10.1128/iai.22.2.350-358.1978.
2
Studies on gonococcus infection. XVII. IgA1-cleaving protease in vaginal washings from women with gonorrhea.
J Infect Dis. 1979 Jan;139(1):89-92. doi: 10.1093/infdis/139.1.89.
3
Purification, characterization, and comparison of the immunoglobulin A1 proteases of Neisseria gonorrhoeae.淋病奈瑟菌免疫球蛋白A1蛋白酶的纯化、特性鉴定及比较
J Bacteriol. 1988 Apr;170(4):1866-73. doi: 10.1128/jb.170.4.1866-1873.1988.
4
Proteolysis of bacterial membrane proteins by Neisseria gonorrhoeae type 2 immunoglobulin A1 protease.淋病奈瑟菌2型免疫球蛋白A1蛋白酶对细菌膜蛋白的蛋白水解作用。
Infect Immun. 1991 Aug;59(8):2535-41. doi: 10.1128/iai.59.8.2535-2541.1991.
5
Immunoglobulin A1 protease types of Neisseria gonorrhoeae and their relationship to auxotype and serovar.淋病奈瑟菌的免疫球蛋白A1蛋白酶类型及其与菌型和血清型的关系。
Infect Immun. 1987 Apr;55(4):931-6. doi: 10.1128/iai.55.4.931-936.1987.
6
Synthetic peptide substrates for the immunoglobulin A1 protease from Neisseria gonorrhoeae (type 2).
Infect Immun. 1991 May;59(5):1818-22. doi: 10.1128/iai.59.5.1818-1822.1991.
7
Attachment to and invasion of human fallopian tube mucosa by an IgA1 protease-deficient mutant of Neisseria gonorrhoeae and its wild-type parent.淋病奈瑟菌的IgA1蛋白酶缺陷突变体及其野生型亲本对人输卵管黏膜的黏附和侵袭。
J Infect Dis. 1984 Nov;150(5):737-44. doi: 10.1093/infdis/150.5.737.
8
Evaluation of immunoglobulin A1 (IgA1) protease and IgA1 protease-inhibitory activity in human female genital infection with Neisseria gonorrhoeae.人类女性淋病奈瑟菌生殖器感染中免疫球蛋白A1（IgA1）蛋白酶及IgA1蛋白酶抑制活性的评估
Infect Immun. 1998 Dec;66(12):5826-32. doi: 10.1128/IAI.66.12.5826-5832.1998.
9
Purification and characterization of an immunoglobulin A1 protease from Bacteroides melaninogenicus.产黑色素拟杆菌免疫球蛋白A1蛋白酶的纯化与特性分析
Infect Immun. 1984 Sep;45(3):550-7. doi: 10.1128/iai.45.3.550-557.1984.
10
Proteases of the pathogenic neisseriae: possible role in infection.致病性奈瑟菌的蛋白酶：在感染中的可能作用。
Microbios. 1986;45(183):113-29.
引用本文的文献
1
Host-directed therapy for bacterial infections -Modulation of the phagolysosome pathway.靶向宿主治疗细菌感染——吞噬体途径的调节。
Front Immunol. 2023 Sep 29;14:1227467. doi: 10.3389/fimmu.2023.1227467. eCollection 2023.
2
Mechanisms of host manipulation by .由……进行宿主操控的机制 。
你提供的原文不完整，“by”后面缺少具体内容。
Front Microbiol. 2023 Feb 3;14:1119834. doi: 10.3389/fmicb.2023.1119834. eCollection 2023.
3
Macrophages in Microbial Pathogenesis: Commonalities of Defense Evasion Mechanisms.微生物发病机制中的巨噬细胞：防御逃避机制的共性。
Infect Immun. 2022 May 19;90(5):e0029121. doi: 10.1128/IAI.00291-21. Epub 2021 Nov 15.
4
Cloning and Expression of H. influenzae 49247 IgA Protease in E. coli.流感嗜血杆菌49247 IgA蛋白酶在大肠杆菌中的克隆与表达
Mol Biotechnol. 2018 Feb;60(2):134-140. doi: 10.1007/s12033-017-0054-3.
5
Pneumococcal IgA1 protease subverts specific protection by human IgA1.肺炎链球菌 IgA1 蛋白酶颠覆了人 IgA1 的特异性保护作用。
Mucosal Immunol. 2014 Mar;7(2):249-56. doi: 10.1038/mi.2013.41. Epub 2013 Jul 3.
6
A bacterial siren song: intimate interactions between Neisseria and neutrophils.细菌的警笛之歌：奈瑟菌与中性粒细胞的亲密互动。
Nat Rev Microbiol. 2012 Jan 31;10(3):178-90. doi: 10.1038/nrmicro2713.
7
Resistance of Neisseria gonorrhoeae to neutrophils.淋病奈瑟菌对中性粒细胞的抗性。
Front Microbiol. 2011 Apr 13;2:77. doi: 10.3389/fmicb.2011.00077. eCollection 2011.
8
Uptake and intra-inclusion accumulation of exogenous immunoglobulin by Chlamydia-infected cells.衣原体感染细胞对外源免疫球蛋白的摄取及包涵体内积累
BMC Microbiol. 2008 Dec 5;8:213. doi: 10.1186/1471-2180-8-213.
9
Genetic and biochemical analysis of gonococcal IgA1 protease: cloning in Escherichia coli and construction of mutants of gonococci that fail to produce the activity.淋球菌IgA1蛋白酶的遗传与生化分析：在大肠杆菌中的克隆及构建无法产生该活性的淋球菌突变体。
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7881-5. doi: 10.1073/pnas.79.24.7881.
10
A surface receptor specific for human IgA on group B streptococci possessing the Ibc protein antigen.具有Ibc蛋白抗原的B族链球菌上的一种对人IgA特异的表面受体。
J Exp Med. 1984 Nov 1;160(5):1467-75. doi: 10.1084/jem.160.5.1467.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
3
The presence of fimbriae (pili) in three species of Neisseria.三种奈瑟菌属细菌中菌毛（纤毛）的存在情况。
J Gen Microbiol. 1971 Feb;65(2):167-73. doi: 10.1099/00221287-65-2-167.
4
Natural and immune human antibodies reactive with antigens of virulent Neisseria gonorrhoeae: immunoglobulins G, M, And A.与致病性淋病奈瑟菌抗原反应的天然和免疫性人类抗体：免疫球蛋白G、M和A。
J Bacteriol. 1967 Jul;94(1):141-8. doi: 10.1128/jb.94.1.141-148.1967.
5
Secretory IgA antibodies in the urethral exudate of men with uncomplicated urethritis due to Neisseria gonorrhoeae.因淋病奈瑟菌导致的非复杂性尿道炎男性患者尿道分泌物中的分泌型IgA抗体。
J Infect Dis. 1973 Jan;127(1):99-101. doi: 10.1093/infdis/127.1.99.
6
Bacteriocins from Neisseria gonorrhoeae and their possible role in epidemiological studies.淋病奈瑟菌产生的细菌素及其在流行病学研究中的可能作用。
J Clin Pathol. 1972 Jan;25(1):60-1. doi: 10.1136/jcp.25.1.60.
7
Growth inhibition among strains of Neisseria gonorrhoeae due to production of inhibitory free fatty acids and lysophosphatidylethanolamine: absence of bacteriocins.淋病奈瑟菌菌株因产生抑制性游离脂肪酸和溶血磷脂酰乙醇胺而导致的生长抑制：不存在细菌素。
Infect Immun. 1974 Sep;10(3):481-8. doi: 10.1128/iai.10.3.481-488.1974.
8
Isolation, properties, and structure of human IgA myeloma globulins.人IgA骨髓瘤球蛋白的分离、特性及结构
Adv Exp Med Biol. 1974;45(0):177-89. doi: 10.1007/978-1-4613-4550-3_20.
9
Paradox of the immune response to uncomplicated gonococcal urethritis.单纯性淋菌性尿道炎免疫反应的悖论
N Engl J Med. 1973 Nov 29;289(22):1170-4. doi: 10.1056/NEJM197311292892205.
10
Quantitative determination of antibody to gonococcal pili. Changes in antibody levels with gonococcal infection.淋菌菌毛抗体的定量测定。淋菌感染时抗体水平的变化。
J Clin Invest. 1973 Nov;52(11):2896-909. doi: 10.1172/JCI107486.
Zotero 插件