Katzman M, Katz R A
Department of Medicine, Milton S. Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey 17033-0850, USA.
Adv Virus Res. 1999;52:371-95. doi: 10.1016/s0065-3527(08)60307-3.
Substrate recognition by the retroviral IN enzyme is critical for retroviral integration. To catalyze this recombination event, IN must recognize and act on two types of substrates, viral DNA and host DNA, yet the necessary interactions exhibit markedly different degrees of specificity. Although particular sequences at the viral DNA termini are recognized by IN, many host DNA sequences can serve as the target for integration. Over the last decade, both in vitro and in vivo data have contributed to our understanding of how IN recognizes its substrates. This review provides an overview of the sequence and structure requirements for recognition of viral and host DNA by different retroviral INs and discusses recent progress in mapping protein domains involved in these interactions.
逆转录病毒整合酶对底物的识别对于逆转录病毒的整合至关重要。为了催化这种重组事件，整合酶必须识别并作用于两种类型的底物，即病毒DNA和宿主DNA，然而必要的相互作用表现出明显不同程度的特异性。尽管整合酶能识别病毒DNA末端的特定序列，但许多宿主DNA序列都可作为整合的靶点。在过去十年中，体外和体内数据都有助于我们理解整合酶如何识别其底物。本综述概述了不同逆转录病毒整合酶识别病毒和宿主DNA的序列和结构要求，并讨论了在绘制参与这些相互作用的蛋白质结构域方面的最新进展。
