Suenaga K, Yokoyama Y, Nishimori I, Sano S, Morita M, Okazaki K, Onishi S
First Department of Internal Medicine, Kochi Medical School, Nankoku, Japan.
Dig Dis Sci. 1999 Jun;44(6):1202-7. doi: 10.1023/a:1026692627460.
Mycobacterium paratuberculosis has been suggested as a causative organism of Crohn's disease. Despite a long-term debate to prove this possibility, the role of this bacteria in the pathogenesis of Crohn's disease is still a subject of controversy. In the present study, serum antibodies (IgG, IgA, and IgM) to the protoplasmic antigen of M. paratuberculosis were quantified in patients with Crohn's disease and in control subjects by using an enzyme-linked immunosorbent assay whose specificity was increased by preabsorbing the sera with cell extracts of Mycobacterium phlei. As compared to normal controls (1/20; 0.062+/-0.022), a significant difference was seen in the antibody-positive prevalence rate and mean values of the serum IgG titer in patients with Crohn's disease (5/13; 0.102+/-0.039) (P < 0.05), but not in patients with ulcerative colitis (2/20; 0.065+/-0.035) and tuberculosis (0/4; 0.053+/-0.008). No significant differences were seen in the antibody-positive prevalence rate and mean values of the serum IgA and IgM titers among the four study groups. These results indicate the unique immune response to M. paratuberculosis in patients with Crohn's disease, suggesting that this organism may play some role in the pathogenesis of Crohn's disease.
副结核分枝杆菌被认为是克罗恩病的致病病原体。尽管为证明这种可能性进行了长期争论，但这种细菌在克罗恩病发病机制中的作用仍然存在争议。在本研究中，通过使用一种酶联免疫吸附测定法对克罗恩病患者和对照受试者血清中针对副结核分枝杆菌原生质抗原的抗体（IgG、IgA和IgM）进行定量，该测定法通过用草分枝杆菌细胞提取物预先吸收血清来提高其特异性。与正常对照（1/20；0.062±0.022）相比，克罗恩病患者的抗体阳性患病率和血清IgG滴度平均值存在显著差异（5/13；0.102±0.039）（P<0.05），但溃疡性结肠炎患者（2/20；0.065±0.035）和结核病患者（0/4；0.053±0.008）中未出现这种差异。四个研究组之间的抗体阳性患病率以及血清IgA和IgM滴度平均值均无显著差异。这些结果表明克罗恩病患者对副结核分枝杆菌存在独特的免疫反应，提示该病原体可能在克罗恩病发病机制中发挥某种作用。
