一种使用重组胸膜肺炎放线杆菌ApxII抗原的新型酶联免疫吸附测定法用于猪群胸膜肺炎的诊断。
A novel enzyme-linked immunosorbent assay using the recombinant Actinobacillus pleuropneumoniae ApxII antigen for diagnosis of pleuropneumonia in pig herds.
作者信息
Leiner G, Franz B, Strutzberg K, Gerlach G F
机构信息
Institut für Mikrobiologie und Tierseuchen, Tierärztliche Hochschule Hannover, 30173 Hannover, Germany.
出版信息
Clin Diagn Lab Immunol. 1999 Jul;6(4):630-2. doi: 10.1128/CDLI.6.4.630-632.1999.
Abstract
For the surveillance of pig herds infected with porcine pleuropneumonia, an enzyme-linked immunosorbent assay (ELISA) using the recombinant Actinobacillus pleuropneumoniae ApxII protein as species- but not serotype-specific antigen was developed. Using this ELISA, 243 of 400 animals from 22 A. pleuropneumoniae-infected herds were classified as seropositive.
摘要
为监测感染猪胸膜肺炎的猪群，开发了一种酶联免疫吸附测定（ELISA），该方法使用重组猪胸膜肺炎放线杆菌ApxII蛋白作为种特异性而非血清型特异性抗原。使用这种ELISA，来自22个感染猪胸膜肺炎放线杆菌的猪群的400头动物中有243头被分类为血清阳性。
相似文献
1
A novel enzyme-linked immunosorbent assay using the recombinant Actinobacillus pleuropneumoniae ApxII antigen for diagnosis of pleuropneumonia in pig herds.
Clin Diagn Lab Immunol. 1999 Jul;6(4):630-2. doi: 10.1128/CDLI.6.4.630-632.1999.
2
Evaluation of an enzyme-linked immunosorbent assay for serological surveillance of infection with Actinobacillus pleuropneumoniae serotype 5 in pig herds.
Vet Microbiol. 2002 Sep 2;88(3):223-32. doi: 10.1016/s0378-1135(02)00111-6.
3
Use of recombinant ApxIV in serodiagnosis of Actinobacillus pleuropneumoniae infections, development and prevalidation of the ApxIV ELISA.
Vet Microbiol. 2004 Apr 19;99(3-4):227-38. doi: 10.1016/j.vetmic.2004.01.004.
4
Lipopolysaccharides of Actinobacillus pleuropneumoniae (serotype 1): a readily obtainable antigen for ELISA serodiagnosis of pig pleuropneumonia.
Vet Microbiol. 1994 Apr;39(3-4):219-30. doi: 10.1016/0378-1135(94)90159-7.
5
Detection of antibodies to Actinobacillus pleuropneumoniae serotype 12 in pig serum using a blocking enzyme-linked immunosorbent assay.
Vet Microbiol. 2002 Oct 2;89(1):61-7. doi: 10.1016/s0378-1135(02)00156-6.
6
Evaluation of a saline boiled extract, capsular polysaccharides and long-chain lipopolysaccharides of Actinobacillus pleuropneumoniae serotype 1 as antigens for the serodiagnosis of swine pleuropneumonia.
Vet Microbiol. 1994 Nov;42(2-3):91-104. doi: 10.1016/0378-1135(94)90009-4.
7
Biochemical characterization of an antigenic saline extract of Actinobacillus pleuropneumoniae serotype 5 and identification of a serotype-specific antigen for ELISA serodiagnosis.
Vet Microbiol. 1992 Mar;30(4):369-85. doi: 10.1016/0378-1135(92)90023-m.
8
Detection of antibodies against Actinobacillus pleuropneumoniae, serotype 2 in porcine colostrum using a blocking enzyme-linked immunosorbent assay specific for serotype 2.
Vet Microbiol. 1995 Mar;43(4):277-81. doi: 10.1016/0378-1135(94)00112-a.
9
10
Evaluation of an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the Apx toxins of Actinobacillus pleuropneumoniae.
Vet Microbiol. 2000 Jan;71(1-2):81-7. doi: 10.1016/s0378-1135(99)00157-1.
引用本文的文献
1
Link between Heterotrophic Carbon Fixation and Virulence in the Porcine Lung Pathogen Actinobacillus pleuropneumoniae.
Infect Immun. 2019 Aug 21;87(9). doi: 10.1128/IAI.00768-18. Print 2019 Sep.
2
Host-pathogen interplay at primary infection sites in pigs challenged with Actinobacillus pleuropneumoniae.
BMC Vet Res. 2017 Feb 28;13(1):64. doi: 10.1186/s12917-017-0979-6.
3
Actinobacillus pleuropneumoniae challenge in swine: diagnostic of lung alterations by infrared thermography.
BMC Vet Res. 2014 Sep 16;10:199. doi: 10.1186/s12917-014-0199-2.
4
Pathway deregulation and expression QTLs in response to Actinobacillus pleuropneumoniae infection in swine.
Mamm Genome. 2014 Dec;25(11-12):600-17. doi: 10.1007/s00335-014-9536-9. Epub 2014 Aug 14.
5
Infrared thermography of the pig thorax: an assessment of selected regions of interest by computed tomographical and anatomical parameters.
Anat Histol Embryol. 2015 Apr;44(2):107-17. doi: 10.1111/ahe.12115. Epub 2014 Apr 9.
6
Identification of QTL affecting resistance/susceptibility to acute Actinobacillus pleuropneumoniae infection in swine.
Mamm Genome. 2014 Apr;25(3-4):180-91. doi: 10.1007/s00335-013-9497-4. Epub 2014 Jan 21.
7
A novel Respiratory Health Score (RHS) supports a role of acute lung damage and pig breed in the course of an Actinobacillus pleuropneumoniae infection.
BMC Vet Res. 2009 Apr 21;5:14. doi: 10.1186/1746-6148-5-14.
8
Analysis of the Actinobacillus pleuropneumoniae ArcA regulon identifies fumarate reductase as a determinant of virulence.
Infect Immun. 2008 Jun;76(6):2284-95. doi: 10.1128/IAI.01540-07. Epub 2008 Mar 31.
9
Use of an Actinobacillus pleuropneumoniae multiple mutant as a vaccine that allows differentiation of vaccinated and infected animals.
Infect Immun. 2006 Jul;74(7):4124-32. doi: 10.1128/IAI.00133-06.
10
Deletion of the anaerobic regulator HlyX causes reduced colonization and persistence of Actinobacillus pleuropneumoniae in the porcine respiratory tract.
Infect Immun. 2005 Aug;73(8):4614-9. doi: 10.1128/IAI.73.8.4614-4619.2005.
本文引用的文献
1
Taxonomy and Serological Identification of Actinobacillus pleuropneumoniae.
Can Vet J. 1988 Jul;29(7):578-80.
2
Prevention and Control of Actinobacillus (Haemophilus) pleuropneumoniae Infection in Swine: A review.
Can Vet J. 1988 Jul;29(7):572-4.
3
Actinobacillus pleuropneumoniae RTX-toxins: uniform designation of haemolysins, cytolysins, pleurotoxin and their genes.
J Gen Microbiol. 1993 Aug;139(8):1723-8. doi: 10.1099/00221287-139-8-1723.
4
Porcine pleuropneumonia.
J Am Vet Med Assoc. 1994 May 1;204(9):1334-40.
5
Lipopolysaccharides of Actinobacillus pleuropneumoniae (serotype 1): a readily obtainable antigen for ELISA serodiagnosis of pig pleuropneumonia.
Vet Microbiol. 1994 Apr;39(3-4):219-30. doi: 10.1016/0378-1135(94)90159-7.
6
Evaluation of a saline boiled extract, capsular polysaccharides and long-chain lipopolysaccharides of Actinobacillus pleuropneumoniae serotype 1 as antigens for the serodiagnosis of swine pleuropneumonia.
Vet Microbiol. 1994 Nov;42(2-3):91-104. doi: 10.1016/0378-1135(94)90009-4.
7
Virulence in Actinobacillus pleuropneumoniae and RTX toxins.
Trends Microbiol. 1995 Jul;3(7):257-61. doi: 10.1016/s0966-842x(00)88939-8.
8
Haemophilus pleuropneumoniae infection in swine: a review.
J Am Vet Med Assoc. 1983 Jun 15;182(12):1331-7.
9
Evaluation of the complement fixation test for the diagnosis of pleuropneumonia of swine caused by Haemophilus pleuropneumoniae.
Can J Comp Med. 1982 Apr;46(2):109-14.
10
An enzyme-linked immunosorbent assay, using an EDTA-extracted antigen for the serology of Haemophilus pleuropneumoniae.
Am J Vet Res. 1981 Dec;42(12):2139-42.
Zotero 插件