Nielsen R
National Veterinary Laboratory, Copenhagen V, Denmark.
Vet Microbiol. 1995 Mar;43(4):277-81. doi: 10.1016/0378-1135(94)00112-a.
A blocking enzyme-linked immunosorbent assay (ELISA) developed for detection of antibodies to Actinobacillus pleuropneumoniae serotype 2 in sera from pigs (Nielsen et al., 1991) was evaluated for its suitability to detect antibodies in colostrum to this serotype. Using colostrum from sows experimentally infected with serotype 2 and from herds known to be infected with this serotype, the sensitivity of the test was 100%. Antibodies to A. pleuropneumoniae serotype 2 could be detected in colostrum of experimentally infected sows until at least 5 days after farrowing. Positive results were not observed with colostrum samples from herds known to be free from A. pleuropneumoniae. The high diagnostic sensitivity and specificity of the assay indicated that screening of herds for A. pleuropneumoniae serotype 2 infection by testing colostrum would be a reliable and simple method for herd monitoring.
为检测猪血清中抗胸膜肺炎放线杆菌2型抗体而开发的一种阻断酶联免疫吸附测定法（ELISA）（Nielsen等人，1991年），对其检测初乳中该血清型抗体的适用性进行了评估。使用来自实验感染2型血清型的母猪以及已知感染该血清型的猪群的初乳，该检测方法的灵敏度为100%。在实验感染的母猪产后至少5天内，其初乳中均可检测到抗胸膜肺炎放线杆菌2型抗体。来自已知无胸膜肺炎放线杆菌的猪群的初乳样本未观察到阳性结果。该检测方法具有较高的诊断灵敏度和特异性，表明通过检测初乳对猪群进行胸膜肺炎放线杆菌2型感染筛查将是一种可靠且简单的猪群监测方法。
