利用单克隆抗体在细胞培养中鉴定I型猪肠道病毒

Identification of group I porcine enteroviruses by monoclonal antibodies in cell culture.

作者信息

Dauber M

机构信息

Federal Research Centre for Virus Diseases of Animals, Friedrich Loeffler Institutes, Institute of Diagnostic Virology, Insel Riems, Germany.

出版信息

Vet Microbiol. 1999 Jun 1;67(1):1-12. doi: 10.1016/s0378-1135(99)00024-3.

DOI:10.1016/s0378-1135(99)00024-3

PMID:10392772

Abstract

A panel of monoclonal antibodies (mAb) against porcine enteroviruses (PEV) was established. One of these mAbs reacts group-specifically with PEV of serotype group I in the indirect immunofluorescence assay (IIF). This mAb is very well suited for diagnosis of PEV infections. However, the mAb neither neutralizes virus nor does it react with virus particles in immuno electron microscopy (IEM). Another mAb is PEV-1 specific in IIF, neutralizes virus, and is suited for IEM. Both mAbs presumably recognize conformation-dependent epitopes of the virus.

摘要

建立了一组针对猪肠道病毒（PEV）的单克隆抗体（mAb）。其中一种单克隆抗体在间接免疫荧光试验（IIF）中与血清型I组的猪肠道病毒发生群特异性反应。这种单克隆抗体非常适合用于诊断猪肠道病毒感染。然而，该单克隆抗体既不能中和病毒，也不能在免疫电子显微镜（IEM）中与病毒颗粒发生反应。另一种单克隆抗体在IIF中具有猪肠道病毒-1特异性，能中和病毒，且适用于IEM。这两种单克隆抗体可能识别病毒的构象依赖性表位。