Santini M T, Rainaldi G
Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, Rome, Italy.
Pathobiology. 1999 May-Jun;67(3):148-57. doi: 10.1159/000028065.
It is becoming more and more apparent that monolayer cultures of tumor cells cannot completely represent the characteristics of three-dimensional solid tumors. Consequently, the multicellular tumor spheroid model, which is of intermediate complexity between in vivo tumors and monolayer cultures, was developed. In this review, the major similarities between spheroids and solid tumors are discussed. After a brief survey of the different spheroid culturing techniques, the general morphological and growth characteristics of these systems are examined and compared to solid tumors. Finally, selected studies regarding the use of tumor spheroids to examine cell response to antineoplastic agents and radiation, cell death including both necrosis and apoptosis and cell adhesion in spheroids are reviewed.
越来越明显的是，肿瘤细胞的单层培养不能完全代表三维实体瘤的特征。因此，开发了多细胞肿瘤球体模型，其复杂性介于体内肿瘤和单层培养之间。在这篇综述中，讨论了球体与实体瘤之间的主要相似之处。在简要概述不同的球体培养技术之后，研究并比较了这些系统的一般形态和生长特征与实体瘤的情况。最后，综述了关于使用肿瘤球体来研究细胞对抗肿瘤药物和辐射的反应、包括坏死和凋亡在内的细胞死亡以及球体中的细胞黏附的选定研究。
