用于区分须癣毛癣菌复合体的几丁质合成酶1基因的种特异性引物。

Species-specific primers of chitin synthase 1 gene for the differentiation of the Trichophyton mentagrophytes complex.

作者信息

Kano R, Nakamura Y, Watari T, Watanabe S, Takahashi H, Tsujimoto H, Hasegawa A

机构信息

Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.

出版信息

Mycoses. 1999 Apr;42(1-2):71-4. doi: 10.1046/j.1439-0507.1999.00265.x.

DOI:10.1046/j.1439-0507.1999.00265.x

PMID:10394852

Abstract

Species-specific primers were designed from nucleotide sequences of the chitin synthase 1 gene (CHS1) of Arthroderma benhamiae, A. simii and A. vanbreuseghemii. The A. benhamiae-specific primer amplified a 560-bp fragment from A. benhamiae but not from A. simii and A. vanbreuseghemii. The A. simii-specific primers amplified a 470-bp fragment from A. simii but not from A. benhamiae and A. vanbreuseghemii. The A. vanbreuseghemii-specific primers amplified a 360-bp fragment from A. vanbreuseghemii but not from A. benhamiae and A. simii. With these species-specific primers of CHS1 genes, 10 clinical isolates from humans and animals were examined by polymerase chain reaction analyses. These isolates proved to be identical to A. vanbreuseghemii.

摘要

根据须癣毛癣菌、猴毛癣菌和万氏毛癣菌几丁质合成酶1基因（CHS1）的核苷酸序列设计了种特异性引物。须癣毛癣菌特异性引物从须癣毛癣菌中扩增出一个560 bp的片段，但从猴毛癣菌和万氏毛癣菌中未扩增出该片段。猴毛癣菌特异性引物从猴毛癣菌中扩增出一个470 bp的片段，但从须癣毛癣菌和万氏毛癣菌中未扩增出该片段。万氏毛癣菌特异性引物从万氏毛癣菌中扩增出一个360 bp的片段，但从须癣毛癣菌和猴毛癣菌中未扩增出该片段。利用这些CHS1基因的种特异性引物，通过聚合酶链反应分析检测了10株来自人和动物的临床分离株。这些分离株被证明与万氏毛癣菌相同。