Kano R, Aihara S, Nakamura Y, Watanabe S, Hasegawa A
Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866, Kameino, Fujisawa, 252-8510, Kanagawa, Japan.
Vet Microbiol. 2001 Jan 5;78(1):85-90. doi: 10.1016/s0378-1135(00)00243-1.
Chitin synthase 1 (Chs1) genes from Microsporum equinum and Trichophyton equinum were compared with those of the other dermatophytes. The Chs1 nucleotide sequences of these dermatophytes from horses showed more than 80% similarity to those of Arthroderma benhamiae, A. fulvum, A. grubyi, A. gypseum, A. incruvatum, A. otae, A. simii, A. vanbreuseghemii, Epidermophyton floccosum, T. mentagrophytes var. interdigitale (T. interdigitale), T. rubrum and T. violaceum. Especially high degree of nucleotide sequence similarity of more than 99% was noted between the Chs1 gene fragments of M. equinum and A. otae, and those of T. equinum, T. interdigitale and A. vanbreuseghemii, respectively. The phylogenetic analysis of their sequences revealed that M. equinum was genetically very close to A. otae and T. equinum to A. vanbreuseghemii. A molecular analysis of Chs1 genes will provide useful information for the genetic relatedness of M. equinum and T. equinum and confirm the value of DNA sequencing in identification of these two dermatophytes.
对来自马小孢子菌和马毛癣菌的几丁质合酶1（Chs1）基因与其他皮肤癣菌的基因进行了比较。这些来自马的皮肤癣菌的Chs1核苷酸序列与贝氏节皮菌、黄曲节皮菌、格鲁比节皮菌、石膏样节皮菌、内卷节皮菌、太田节皮菌、西氏节皮菌、范布节皮菌、絮状表皮癣菌、指间毛癣菌变种（指间毛癣菌）、红色毛癣菌和紫色毛癣菌的Chs1核苷酸序列相似度超过80%。尤其值得注意的是，马小孢子菌与太田节皮菌的Chs1基因片段之间，以及马毛癣菌、指间毛癣菌与范布节皮菌的Chs1基因片段之间，核苷酸序列相似度分别超过99%。对它们序列的系统发育分析表明，马小孢子菌在基因上与太田节皮菌非常接近，马毛癣菌与范布节皮菌非常接近。对Chs1基因的分子分析将为马小孢子菌和马毛癣菌的遗传相关性提供有用信息，并证实DNA测序在鉴定这两种皮肤癣菌中的价值。
