Kano R, Nakamura Y, Yasuda K, Watari T, Watanabe S, Takahashi H, Tsujimoto H, Hasegawa A
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Microbiol Immunol. 1998;42(8):575-8. doi: 10.1111/j.1348-0421.1998.tb02326.x.
A clinical isolate of Trichophyton mentagrophytes from rabbit was examined by polymerase chain reaction (PCR) analysis and a mating experiment. The species-specific primers designed from the nucleotide sequences of the chitin synthase 1 (CHS1) gene in the teleomorph of Arthroderma benhamiae amplified a fragment from genomic DNA samples of A. benhamiae and the clinical isolate but not from those of A. simii and A. vanbreuseghemii. On the other hand, the species-specific primers of A. simii and A. vanbreuseghemii did not amplify any fragment from the genomic DNA of the clinical isolates. When the isolate was respectively crossed with (+) or (-) tester strains of A. benhamiae, A. simii and A. vanbreuseghemii, ascospores were produced in the crossing with the A. benhamiae (+) strain. Therefore, the isolate was identified to be A. benhamiae (-), confirming the result of molecular analysis. This is the first report on the isolation of A. benhamiae in Japan.
通过聚合酶链反应（PCR）分析和交配实验，对从兔子身上分离出的须癣毛癣菌临床菌株进行了检测。从巴西木霉有性型几丁质合酶1（CHS1）基因的核苷酸序列设计的种特异性引物，能从巴西木霉和该临床分离株的基因组DNA样本中扩增出片段，但不能从西氏木霉和范布列塞姆木霉的样本中扩增出片段。另一方面，西氏木霉和范布列塞姆木霉的种特异性引物未从临床分离株的基因组DNA中扩增出任何片段。当该分离株分别与巴西木霉、西氏木霉和范布列塞姆木霉的（+）或（-）测试菌株杂交时，与巴西木霉（+）菌株杂交产生了子囊孢子。因此，该分离株被鉴定为巴西木霉（-），证实了分子分析结果。这是日本首次关于巴西木霉分离的报告。
