利用几丁质合成酶1基因序列对8种皮肤癣菌进行系统发育分析。

Phylogenetic analysis of 8 dermatophyte species using chitin synthase 1 gene sequences.

作者信息

Kano R, Nakamura Y, Watari T, Watanabe S, Takahashi H, Tsujimoto H, Hasegawa A

机构信息

Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.

出版信息

Mycoses. 1997 Dec;40(11-12):411-4. doi: 10.1111/j.1439-0507.1997.tb00176.x.

DOI:10.1111/j.1439-0507.1997.tb00176.x

PMID:9470404

Abstract

Nucleotide sequences of chitin synthase 1 (CHS1) gene of eight species of dermatophytes, Arthroderma benhamiae, A. fulvum, A. grubyi, A. gypseum, A. incurvatum, A. otae, A. simii and A. vanbreuseghemii were obtained and analysed for their phylogenetic relationship. A 600-bp genomic DNA fragment of the CHS1 gene was amplified from these dermatophytes by polymerase chain reaction (PCR) and sequenced. The CHS1 nucleotide sequences of these eight dermatophyte species showed more than 85% similarity between the species. The phylogenetic analysis of their sequences revealed three clusters, the first cluster consisting of A. benhamiae, A. simii and A. vanbreuseghemii, the second cluster consisting of A. fulvum, A. gypseum and A. incurvatum, and the third cluster consisting of A. grubyi and A. otae. The phylogenetic analysis of CHS1 gene in this study will provide useful information for classification and understanding the evolution of these dermatophyte species.

摘要

获取了八种皮肤癣菌（即贝氏节皮菌、黄曲霉、格鲁比节皮菌、石膏样小孢子菌、弯曲节皮菌、太田节皮菌、西氏节皮菌和范布勒节皮菌）几丁质合酶1（CHS1）基因的核苷酸序列，并对其系统发育关系进行了分析。通过聚合酶链反应（PCR）从这些皮肤癣菌中扩增出CHS1基因的一个600碱基对的基因组DNA片段并进行测序。这八种皮肤癣菌的CHS1核苷酸序列在种间显示出超过85%的相似性。对其序列的系统发育分析揭示了三个聚类，第一类由贝氏节皮菌、西氏节皮菌和范布勒节皮菌组成，第二类由黄曲霉、石膏样小孢子菌和弯曲节皮菌组成，第三类由格鲁比节皮菌和太田节皮菌组成。本研究中CHS1基因的系统发育分析将为这些皮肤癣菌种的分类和进化理解提供有用信息。