鸡卵黄免疫球蛋白（IgY）在用于检测非洲马瘟病毒的双抗体夹心酶联免疫吸附测定（ELISA）中的应用。

The use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus.

作者信息

Du Plessis D H, Van Wyngaardt W, Romito M, Du Plessis M, Maree S

机构信息

Immunology Division, Onderstepoort Veterinary Institute, South Africa.

出版信息

Onderstepoort J Vet Res. 1999 Mar;66(1):25-8.

PMID:10396758

Abstract

An indirect sandwich ELISA that can detect as little as 8 ng of African horsesickness virus (AHSV) was developed. Viral antigen was captured from suspension using an immobilized monoclonal antibody specific for an epitope on VP7, a protein that is a major constituent of the virus core. Egg-yolk derived chicken IgY directed against AHSV (serotype 3) was used as the secondary antibody. Since IgY and mouse IgG do not cross-react serologically, the secondary antibody was not labelled, but was instead detected with enzyme-coupled sheep antibodies directed against avian immunoglobulins. The assay recognized all nine AHSV serotypes, but not the Cascara isolate of equine encephalosis virus, a related orbivirus that also infects horses. In addition to being able to detect and quantify whole AHSV, the ELISA could show the presence of VP7 produced by recombinant baculoviruses.

摘要

开发了一种间接夹心酶联免疫吸附测定法（ELISA），该方法能够检测低至8纳克的非洲马瘟病毒（AHSV）。使用针对病毒核心主要成分VP7上一个表位的固定化单克隆抗体，从悬浮液中捕获病毒抗原。针对AHSV（血清型3）的蛋黄源鸡IgY用作二抗。由于IgY和小鼠IgG在血清学上不发生交叉反应，二抗未进行标记，而是用针对禽免疫球蛋白的酶联羊抗体进行检测。该测定法可识别所有9种AHSV血清型，但不能识别马脑脊髓炎病毒的卡斯卡拉分离株，后者是一种也感染马的相关环状病毒。除了能够检测和定量完整的AHSV外，该ELISA还能显示重组杆状病毒产生的VP7的存在。