荷兰和瑞士腹泻仔猪中产气荚膜梭菌β2毒素基因的检测

Detection of the beta2 toxin gene of Clostridium perfringens in diarrhoeic piglets in The Netherlands and Switzerland.

作者信息

Klaasen H L, Molkenboer M J, Bakker J, Miserez R, Häni H, Frey J, Popoff M R, van den Bosch J F

机构信息

Department of Bacteriological Research and Development, Intervet International BV, Boxmeer, The Netherlands.

出版信息

FEMS Immunol Med Microbiol. 1999 Jul;24(3):325-32. doi: 10.1111/j.1574-695X.1999.tb01301.x.

DOI:10.1111/j.1574-695X.1999.tb01301.x

PMID:10397318

Abstract

The two studies presented here were done to determine the prevalence of the alpha, beta, epsilon and enterotoxin genes and the novel beta2 toxin gene of Clostridium perfringens in neonatal or pre-weaned piglets with diarrhoea or necrotic enteritis. All C. perfringens isolates were positive for the alpha and negative for the epsilon and enterotoxin gene, implying that only non-enterotoxigenic type A and C strains were detected. The most important findings were the relatively high prevalence of the beta2 toxin gene in isolates from diarrhoeic piglets in both studies, and, in one of the two studies, absence of strains with only the alpha and beta toxin gene. These data are supportive for the suggestion of a causal relationship of beta2 toxin-producing strains with digestive tract diseases in piglets.

摘要

这里展示的两项研究旨在确定产气荚膜梭菌的α、β、ε和肠毒素基因以及新型β2毒素基因在患有腹泻或坏死性肠炎的新生或断奶前仔猪中的流行情况。所有产气荚膜梭菌分离株的α基因均呈阳性，ε和肠毒素基因呈阴性，这意味着仅检测到非产肠毒素的A型和C型菌株。最重要的发现是，在两项研究中，腹泻仔猪分离株中β2毒素基因的流行率相对较高，并且在两项研究中的一项中，不存在仅携带α和β毒素基因的菌株。这些数据支持了产生β2毒素的菌株与仔猪消化道疾病之间存在因果关系的观点。