Wong S W, Rangan A M, Bilous A M, Boyages J, Gebski V, Benson E M
Department of Immunopathology, New South Wales Breast Cancer Institute, Australia.
Pathology. 1999 May;31(2):90-4. doi: 10.1080/003130299105241.
This study aimed to determine the prognostic significance of DNA ploidy and S-phase fraction (SPF) measurements in our laboratory for patients with node-negative breast cancer. Frozen tumors from axillary node-negative breast cancer patients (n = 50) treated at Westmead Hospital, NSW, between 1988 and 1991 were analysed by flow cytometry. The median duration of follow-up for all patients was 8.4 years. Forty-six specimens provided evaluable DNA histograms with 43% (n = 20) diploid and 56% (n = 26) aneuploid tumors identified. Comparisons of DNA ploidy status and SPF were made with traditional prognostic variables, which included age, menopausal status, tumor size, histologic grade and hormone receptor status. Our results showed that there was no significant difference in disease-free or overall survival between patients with diploid and aneuploid tumors. Histologic grade 3 tumors were more likely to be aneuploid and had higher SPF than grade 1 or 2 tumors. Patients with grade 3 tumors and a high SPF were four times more likely to relapse than the rest of the population. These results indicate that DNA flow cytometric analysis in our laboratory provides additional prognostic data that could be utilised alongside traditional clinical and histopathologic indicators for predicting outcome for patients.
本研究旨在确定在我们实验室中，DNA倍体和S期细胞分数（SPF）测量对腋淋巴结阴性乳腺癌患者的预后意义。对1988年至1991年间在新南威尔士州韦斯特米德医院接受治疗的腋淋巴结阴性乳腺癌患者（n = 50）的冰冻肿瘤进行了流式细胞术分析。所有患者的中位随访时间为8.4年。46份标本提供了可评估的DNA直方图，其中43%（n = 20）为二倍体肿瘤，56%（n = 26）为非整倍体肿瘤。将DNA倍体状态和SPF与传统预后变量进行了比较，传统预后变量包括年龄、绝经状态、肿瘤大小、组织学分级和激素受体状态。我们的结果表明，二倍体肿瘤患者和非整倍体肿瘤患者在无病生存期或总生存期方面没有显著差异。组织学3级肿瘤比1级或2级肿瘤更可能是非整倍体，且SPF更高。3级肿瘤且SPF高的患者复发的可能性是其他患者的四倍。这些结果表明，我们实验室的DNA流式细胞术分析提供了额外的预后数据，可与传统的临床和组织病理学指标一起用于预测患者的预后。
